Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC9A2 / NHE2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99948-100
Catalog Number: LS-G99948
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC9A2 (NM_003048), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Na(+)/H(+) exchanger 2 | NHE-2 | Sodium-hydrogen exchanger 2 | Sodium/hydrogen exchanger 2 | NHE2 | SLC9A2
- NCBI GenBank
- NM_003048
- SwissProt
- Q9UBY0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPLGNWRSL RAPLPPMLLL LLLQVAGPVG ALAETLLNAP RAMGTSSSPP SPASVVAPGT61TLFEESRLPV FTLDYPHVQI PFEITLWILL ASLAKIGFHL YHKLPTIVPE SCLLIMVGLL121LGGIIFGVDE KSPPAMKTDV FFLYLLPPIV LDAGYFMPTR PFFENIGTIF WYAVVGTLWN181SIGIGVSLFG ICQIEAFGLS DITLLQNLLF GSLISAVDPV AVLAVFENIH VNEQLYILVF241GESLLNDAVT VVLYNLFKSF CQMKTIETID VFAGIANFFV VGIGGVLIGI FLGFIAAFTT301RFTHNIRVIE PLFVFLYSYL SYITAEMFHL SGIMAITACA MTMNKYVEEN VSQKSYTTIK361YFMKMLSSVS ETLIFIFMGV STVGKNHEWN WAFVCFTLAF CLMWRALGVF VLTQVINRFR421TIPLTFKDQF IIAYGGLRGA ICFALVFLLP AAVFPRKKLF ITAAIVVIFF TVFILGITIR481PLVEFLDVKR SNKKQQAVSE EIYCRLFDHV KTGIEDVCGH WGHNFWRDKF KKFDDKYLRK541LLIRENQPKS SIVSLYKKLE IKHAIEMAET GMISTVPTFA SLNDCREEKI RKVTSSETDE601IRELLSRNLY QIRQRTLSYN RHSLTADTSE RQAKEILIRR RHSLRESIRK DSSLNREHRA661STSTSRYLSL PKNTKLPEKL QKRRTISIAD GNSSDSDADA GTTVLNLQPR ARRFLPEQFS721KKSPQSYKME WKNEVDVDSG RDMPSTPPTP HSREKGTQTS GLLQQPLLSK DQSGSEREDS781LTEGIPPKPP PRLVWRASEP GSRKARFGSE KPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
812 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.