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Human SLC9A2 / NHE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SLC9A2 / NHE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SLC9A2 antibodies →
$220.00each
Catalog Number: LS-G89158-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SLC9A2 (NM_003048) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Na(+)/H(+) exchanger 2 | NHE-2 | Sodium-hydrogen exchanger 2 | Sodium/hydrogen exchanger 2 | NHE2 | SLC9A2
Origin Species
Human
NCBI GenBank
NM_003048
SwissProt
Q9UBY0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPLGNWRSL RAPLPPMLLL LLLQVAGPVG ALAETLLNAP RAMGTSSSPP SPASVVAPGT
61TLFEESRLPV FTLDYPHVQI PFEITLWILL ASLAKIGFHL YHKLPTIVPE SCLLIMVGLL
121LGGIIFGVDE KSPPAMKTDV FFLYLLPPIV LDAGYFMPTR PFFENIGTIF WYAVVGTLWN
181SIGIGVSLFG ICQIEAFGLS DITLLQNLLF GSLISAVDPV AVLAVFENIH VNEQLYILVF
241GESLLNDAVT VVLYNLFKSF CQMKTIETID VFAGIANFFV VGIGGVLIGI FLGFIAAFTT
301RFTHNIRVIE PLFVFLYSYL SYITAEMFHL SGIMAITACA MTMNKYVEEN VSQKSYTTIK
361YFMKMLSSVS ETLIFIFMGV STVGKNHEWN WAFVCFTLAF CLMWRALGVF VLTQVINRFR
421TIPLTFKDQF IIAYGGLRGA ICFALVFLLP AAVFPRKKLF ITAAIVVIFF TVFILGITIR
481PLVEFLDVKR SNKKQQAVSE EIYCRLFDHV KTGIEDVCGH WGHNFWRDKF KKFDDKYLRK
541LLIRENQPKS SIVSLYKKLE IKHAIEMAET GMISTVPTFA SLNDCREEKI RKVTSSETDE
601IRELLSRNLY QIRQRTLSYN RHSLTADTSE RQAKEILIRR RHSLRESIRK DSSLNREHRA
661STSTSRYLSL PKNTKLPEKL QKRRTISIAD GNSSDSDADA GTTVLNLQPR ARRFLPEQFS
721KKSPQSYKME WKNEVDVDSG RDMPSTPPTP HSREKGTQTS GLLQQPLLSK DQSGSEREDS
781LTEGIPPKPP PRLVWRASEP GSRKARFGSE KP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

812 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.