Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC6A9 / GLYT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114976-100
Catalog Number: LS-G114976
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Glyt1(SLC6A9) (NM_006934), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GLYT1 | Glycine transporter 1 | GlyT-1 | SLC6A9 | type 1
- Origin Species
- Human
- NCBI GenBank
- NM_006934
- SwissProt
- P48067
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGGDTRAAI ARPRMAAAHG PVAPSSPEQN GAVPSEATKR DQNLKRGNWG NQIEFVLTSV61GYAVGLGNVW RFPYLCYRNG GGAFMFPYFI MLIFCGIPLF FMELSFGQFA SQGCLGVWRI121SPMFKGVGYG MMVVSTYIGI YYNVVICIAF YYFFSSMTHV LPWAYCNNPW NTHDCAGVLD181ASNLTNGSRP AALPSNLSHL LNHSLQRTSP SEEYWRLYVL KLSDDIGNFG EVRLPLLGCL241GVSWLVVFLC LIRGVKSSGK VVYFTATFPY VVLTILFVRG VTLEGAFDGI MYYLTPQWDK301ILEAKVWGDA ASQIFYSLGC AWGGLITMAS YNKFHNNCYR DSVIISITNC ATSVYAGFVI361FSILGFMANH LGVDVSRVAD HGPGLAFVAY PEALTLLPIS PLWSLLFFFM LILLGLGTQF421CLLETLVTAI VDEVGNEWIL QKKTYVTLGV AVAGFLLGIP LTSQAGIYWL LLMDNYAASF481SLVVISCIMC VAIMYIYGHR NYFQDIQMML GFPPPLFFQI CWRFVSPAII FFILVFTVIQ541YQPITYNHYQ YPGWAVAIGF LMALSSVLCI PLYAMFRLCR TDGDTLLQRL KNATKPSRDW601GPALLEHRTG RYAPTIAPSP EDGFEVQPLH PDKAQIPIVG SNGSSRLQDS RITRTRPLEQKLISEEDLAANDILDYKDDDDKV
652 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.