Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC6A9 / GLYT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121087-100
Catalog Number: LS-G121087
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Glyt1(SLC6A9) (NM_001024845), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GLYT1 | Glycine transporter 1 | GlyT-1 | SLC6A9 | type 1
- Origin Species
- Human
- NCBI GenBank
- NM_006934
- SwissProt
- P48067
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVGKGAKGML NGAVPSEATK RDQNLKRGNW GNQIEFVLTS VGYAVGLGNV WRFPYLCYRN61GGGAFMFPYF IMLIFCGIPL FFMELSFGQF ASQGCLGVWR ISPMFKGVGY GMMVVSTYIG121IYYNVVICIA FYYFFSSMTH VLPWAYCNNP WNTHDCAGVL DASNLTNGSR PAALPSNLSH181LLNHSLQRTS PSEEYWRLYV LKLSDDIGNF GEVRLPLLGC LGVSWLVVFL CLIRGVKSSG241KVVYFTATFP YVVLTILFVR GVTLEGAFDG IMYYLTPQWD KILEAKVWGD AASQIFYSLG301CAWGGLITMA SYNKFHNNCY RDSVIISITN CATSVYAGFV IFSILGFMAN HLGVDVSRVA361DHGPGLAFVA YPEALTLLPI SPLWSLLFFF MLILLGLGTQ FCLLETLVTA IVDEVGNEWI421LQKKTYVTLG VAVAGFLLGI PLTSQAGIYW LLLMDNYAAS FSLVVISCIM CVAIMYIYGH481RNYFQDIQMM LGFPPPLFFQ ICWRFVSPAI IFFILVFTVI QYQPITYNHY QYPGWAVAIG541FLMALSSVLC IPLYAMFRLC RTDGDTLLQR LKNATKPSRD WGPALLEHRT GRYAPTIAPS601PEDGFEVQPL HPDKAQIPIV GSNGSSRLQD SRITRTRPLEQKLISEEDLAANDILDYKDDDDKV
633 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.