Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC5A2 / SGLT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117592-100
Catalog Number: LS-G117592
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SGLT2(SLC5A2) (NM_003041), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Na(+)/glucose cotransporter 2 | SGLT2 | SLC5A2 | Sodium-glucose transporter 2 | Sodium/glucose cotransporter 2
- Origin Species
- Human
- NCBI GenBank
- NM_003041
- SwissProt
- P31639
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEHTEAGSA PEMGAQKALI DNPADILVIA AYFLLVIGVG LWSMCRTNRG TVGGYFLAGR61SMVWWPVGAS LFASNIGSGH FVGLAGTGAA SGLAVAGFEW NALFVVLLLG WLFAPVYLTA121GVITMPQYLR KRFGGRRIRL YLSVLSLFLY IFTKISVDMF SGAVFIQQAL GWNIYASVIA181LLGITMIYTV TGGLAALMYT DTVQTFVILG GACILMGYAF HEVGGYSGLF DKYLGAATSL241TVSEDPAVGN ISSFCYRPRP DSYHLLRHPV TGDLPWPALL LGLTIVSGWY WCSDQVIVQR301CLAGKSLTHI KAGCILCGYL KLTPMFLMVM PGMISRILYP DEVACVVPEV CRRVCGTEVG361CSNIAYPRLV VKLMPNGLRG LMLAVMLAAL MSSLASIFNS SSTLFTMDIY TRLRPRAGDR421ELLLVGRLWV VFIVVVSVAW LPVVQAAQGG QLFDYIQAVS SYLAPPVSAV FVLALFVPRV481NEQGAFWGLI GGLLMGLARL IPEFSFGSGS CVQPSACPAF LCGVHYLYFA IVLFFCSGLL541TLTVSLCTAP IPRKHLHRLV FSLRHSKEER EDLDADEQQG SSLPVQNGCP ESAMEMNEPQ601APAPSLFRQC LLWFCGMSRG GVGSPPPLTQ EEAAAAARRL EDISEDPSWA RVVNLNALLM661MAVAVFLWGF YATRTRPLEQKLISEEDLAANDILDYKDDDDKV
672 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.