Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC2A13 / HMIT / GLUT13 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129076-100
Catalog Number: LS-G129076
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC2A13 (NM_052885), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- H(+)-myo-inositol symporter | HMIT | Glut13 | SLC2A13
- Origin Species
- Human
- NCBI GenBank
- NM_052885
- SwissProt
- Q96QE2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRKASENVE YTLRSLSSLM GERRRKQPEP DAASAAGECS LLAAAESSTS LQSAGAGGGG61VGDLERAARR QFQQDETPAF VYVVAVFSAL GGFLFGYDTG VVSGAMLLLK RQLSLDALWQ121ELLVSSTVGA AAVSALAGGA LNGVFGRRAA ILLASALFTA GSAVLAAANN KETLLAGRLV181VGLGIGIASM TVPVYIAEVS PPNLRGRLVT INTLFITGGQ FFASVVDGAF SYLQKDGWRY241MLGLAAVPAV IQFFGFLFLP ESPRWLIQKG QTQKARRILS QMRGNQTIDE EYDSIKNNIE301EEEKEVGSAG PVICRMLSYP PTRRALIVGC GLQMFQQLSG INTIMYYSAT ILQMSGVEDD361RLAIWLASVT AFTNFIFTLV GVWLVEKVGR RKLTFGSLAG TTVALIILAL GFVLSAQVSP421RITFKPIAPS GQNATCTRYS YCNECMLDPD CGFCYKMNKS TVIDSSCVPV NKASTNEAAW481GRCENETKFK TEDIFWAYNF CPTPYSWTAL LGLILYLVFF APGMGPMPWT VNSEIYPLWA541RSTGNACSSG INWIFNVLVS LTFLHTAEYL TYYGAFFLYA GFAAVGLLFI YGCLPETKGK601KLEEIESLFD NRLCTCGTSD SDEGRYIEYI RVKGSNYHLS DNDASDVETRTRPLEQKLISEEDLAANDILDYKDDDDKV
648 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.