Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC22A7 / OAT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100881-100
Catalog Number: LS-G100881
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC22A7 (NM_006672), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HOAT2 | Liver-specific transporter | OAT2 | Organic anion transporter 2 | SLC22A7 | NLT | Novel liver transporter
- Origin Species
- Human
- NCBI GenBank
- NM_006672
- SwissProt
- Q9Y694
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGFEELLEQV GGFGPFQLRN VALLALPRVL LPLHFLLPIF LAAVPAHRCA LPGAPANFSH61QDVWLEAHLP REPDGTLSSC LRFAYPQALP NTTLGEERQS RGELEDEPAT VPCSQGWEYD121HSEFSSTIAT ESQWDLVCEQ KGLNRAASTF FFAGVLVGAV AFGYLSDRFG RRRLLLVAYV181STLVLGLASA ASVSYVMFAI TRTLTGSALA GFTIIVMPLE LEWLDVEHRT VAGVLSSTFW241TGGVMLLALV GYLIRDWRWL LLAVTLPCAP GILSLWWVPE SARWLLTQGH VKEAHRYLLH301CARLNGRPVC EDSFSQEAVS KVAAGERVVR RPSYLDLFRT PRLRHISLCC VVVWFGVNFS361YYGLSLDVSG LGLNVYQTQL LFGAVELPSK LLVYLSVRYA GRRLTQAGTL LGTALAFGTR421LLVSSDMKSW STVLAVMGKA FSEAAFTTAY LFTSELYPTV LRQTGMGLTA LVGRLGGSLA481PLAALLDGVW LSLPKLTYGG IALLAAGTAL LLPETRQAQL PETIQDVERK SAPTSLQEEE541MPMKQVQNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
548 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.