Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC22A15 / FLIPT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101567-100
Catalog Number: LS-G101567
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC22A15 (NM_018420), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLIPT1 | Trans-like protein | Flipt 1 | PRO34686 | SLC22A15
- Origin Species
- Human
- NCBI GenBank
- AY145501
- SwissProt
- Q8IZD6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGIYQMYLCF LLAVLLQLYV ATEAILIALV GATPSYHWDL AELLPNQSHG NQSAGEDQAF61GDWLLTANGS EIHKHVHFSS SFTSIASEWF LIANRSYKVS AASSFFFSGV FVGVISFGQL121SDRFGRKKVY LTGFALDILF AIANGFSPSY EFFAVTRFLV GMMNGGMSLV AFVLLNECVG181TAYWALAGSI GGLFFAVGIA QYALLGYFIR SWRTLAILVN LQGTVVFLLS LFIPESPRWL241YSQGRLSEAE EALYLIAKRN RKLKCTFSLT HPANRSCRET GSFLDLFRYR VLLGHTLILM301FIWFVCSLVY YGLTLSAGDL GGSIYANLAL SGLIEIQSYP LCIYLINQKW FGRKRTLSAF361LCLGGLACLI VMFLPEKKDT GVFAVVNSHS LSLLGKLTIS AAFNIVYIYT SELYPTVIRN421VGLGTCSMFS RVGGIIAPFI PSLKYVQWSL PFIVFGATGL TSGLLSLLLP ETLNSPLLET481FSDLQVYSYR RLGEEALSLQ ALDPQQCVDK ESSLGSESEE EEEFYDADEE TQMIKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
535 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.