LSBio
Human SLC22A15 / FLIPT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SLC22A15 / FLIPT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G92470-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SLC22A15 (NM_018420) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FLIPT1 | Trans-like protein | Flipt 1 | PRO34686 | SLC22A15
Origin Species
Human
NCBI GenBank
AY145501
SwissProt
Q8IZD6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGIYQMYLCF LLAVLLQLYV ATEAILIALV GATPSYHWDL AELLPNQSHG NQSAGEDQAF
61GDWLLTANGS EIHKHVHFSS SFTSIASEWF LIANRSYKVS AASSFFFSGV FVGVISFGQL
121SDRFGRKKVY LTGFALDILF AIANGFSPSY EFFAVTRFLV GMMNGGMSLV AFVLLNECVG
181TAYWALAGSI GGLFFAVGIA QYALLGYFIR SWRTLAILVN LQGTVVFLLS LFIPESPRWL
241YSQGRLSEAE EALYLIAKRN RKLKCTFSLT HPANRSCRET GSFLDLFRYR VLLGHTLILM
301FIWFVCSLVY YGLTLSAGDL GGSIYANLAL SGLIEIQSYP LCIYLINQKW FGRKRTLSAF
361LCLGGLACLI VMFLPEKKDT GVFAVVNSHS LSLLGKLTIS AAFNIVYIYT SELYPTVIRN
421VGLGTCSMFS RVGGIIAPFI PSLKYVQWSL PFIVFGATGL TSGLLSLLLP ETLNSPLLET
481FSDLQVYSYR RLGEEALSLQ ALDPQQCVDK ESSLGSESEE EEEFYDADEE TQMIK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

535 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.