Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC10A1 / NTCP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117584-100
Catalog Number: LS-G117584
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC10A1 (NM_003049), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Growth-inhibiting protein 29 | Na(+)/bile acid cotransporter | Sodium/bile acid cotransporter | Na+/bile acid cotransporter | NTCP | NTCP1 | SLC10A1
- Origin Species
- Human
- NCBI GenBank
- NM_003049
- SwissProt
- Q14973
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAHNASAPF NFTLPPNFGK RPTDLALSVI LVFMLFFIML SLGCTMEFSK IKAHLWKPKG61LAIALVAQYG IMPLTAFVLG KVFRLKNIEA LAILVCGCSP GGNLSNVFSL AMKGDMNLSI121VMTTCSTFCA LGMMPLLLYI YSRGIYDGDL KDKVPYKGIV ISLVLVLIPC TIGIVLKSKR181PQYMRYVIKG GMIIILLCSV AVTVLSAINV GKSIMFAMTP LLIATSSLMP FIGFLLGYVL241SALFCLNGRC RRTVSMETGC QNVQLCSTIL NVAFPPEVIG PLFFFPLLYM IFQLGEGLLL301IAIFWCYEKF KTPKDKTKMI YTAATTEETI PGALGNGTYK GEDCSPCTASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
349 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.