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SIGLEC11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SIGLEC11 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SIGLEC11 antibodies →
$504.00each
Catalog Number: LS-G125411-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIGLEC11 (NM_001135163), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Sialic acid-binding lectin 11 | Siglec-11 | SIGLEC11
Origin Species
Human
SwissProt
Q96RL6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVPGQAQPQS PEMLLLPLLL PVLGAGSLNK DPSYSLQVQR QVPVPEGLCV IVSCNLSYPR
61DGWDESTAAY GYWFKGRTSP KTGAPVATNN QSREVEMSTR DRFQLTGDPG KGSCSLVIRD
121AQREDEAWYF FRVERGSRVR HSFLSNAFFL KVTALTKKPD VYIPETLEPG QPVTVICVFN
181WAFKKCPAPS FSWTGAALSP RRTRPSTSHF SVLSFTPSPQ DHDTDLTCHV DFSRKGVSAQ
241RTVRLRVAYA PKDLIISISH DNTSALELQG NVIYLEVQKG QFLRLLCAAD SQPPATLSWV
301LQDRVLSSSH PWGPRTLGLE LRGVRAGDSG RYTCRAENRL GSQQQALDLS VQYPPENLRV
361MVSQANRTVL ENLGNGTSLP VLEGQSLRLV CVTHSSPPAR LSWTRWGQTV GPSQPSDPGV
421LELPPIQMEH EGEFTCHAQH PLGSQHVSLS LSVHWKLEHG GGLGLGAALG AGVAALLAFC
481SCLVVFRVKI CRKEARKRAA AEQDVPSTLG PISQGHQHEC SAGSSQDHPP PGAATYTPGK
541GEEQELHYAS LSFQGLRLWE PADQEAPSTT EYSEIKIHTG QPLRGPGFGL QLEREMSGMV
601PK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

602 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.