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Human SIGLEC11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SIGLEC11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SIGLEC11 antibodies →
$220.00each
Catalog Number: LS-G92629-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SIGLEC11 (NM_052884) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Sialic acid-binding lectin 11 | Siglec-11 | SIGLEC11
Origin Species
Human
SwissProt
Q96RL6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MVTAPPPGFL LEEFPSQPGR PRSQMVPGQA QPQSPEMLLL PLLLPVLGAG SLNKDPSYSL
61QVQRQVPVPE GLCVIVSCNL SYPRDGWDES TAAYGYWFKG RTSPKTGAPV ATNNQSREVE
121MSTRDRFQLT GDPGKGSCSL VIRDAQREDE AWYFFRVERG SRVRHSFLSN AFFLKVTALT
181KKPDVYIPET LEPGQPVTVI CVFNWAFKKC PAPSFSWTGA ALSPRRTRPS TSHFSVLSFT
241PSPQDHDTDL TCHVDFSRKG VSAQRTVRLR VAYAPKDLII SISHDNTSAL ELQGNVIYLE
301VQKGQFLRLL CAADSQPPAT LSWVLQDRVL SSSHPWGPRT LGLELRGVRA GDSGRYTCRA
361ENRLGSQQQA LDLSVQYPPE NLRVMVSQAN RTVLENLGNG TSLPVLEGQS LRLVCVTHSS
421PPARLSWTRW GQTVGPSQPS DPGVLELPPI QMEHEGEFTC HAQHPLGSQH VSLSLSVHYP
481PQLLGPSCSW EAEGLHCSCS SQASPAPSLR WWLGEELLEG NSSQGSFEVT PSSAGPWANS
541SLSLHGGLSS GLRLRCKAWN VHGAQSGSVF QLLPGKLEHG GGLGLGAALG AGVAALLAFC
601SCLVVFRVKI CRKEARKRAA AEQDVPSTLG PISQGHQHEC SAGSSQDHPP PGAATYTPGK
661GEEQELHYAS LSFQGLRLWE PADQEAPSTT EYSEIKIHTG QPLRGPGFGL QLEREMSGMV
721PK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

722 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.