Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SHH / Sonic Hedgehog Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G123337-100
Catalog Number: LS-G123337
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sonic Hedgehog(SHH) (NM_000193), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HHG-1 | HLP3 | Sonic hedgehog | Sonic hedgehog homolog | MCOPCB5 | SHH | Sonic hedgehog protein | HHG1 | HPE3 | SMMCI | TPT | TPTPS
- Origin Species
- Human
- NCBI GenBank
- NM_000193
- SwissProt
- Q15465
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEMLLLARC LLLVLVSSLL VCSGLACGPG RGFGKRRHPK KLTPLAYKQF IPNVAEKTLG61ASGRYEGKIS RNSERFKELT PNYNPDIIFK DEENTGADRL MTQRCKDKLN ALAISVMNQW121PGVKLRVTEG WDEDGHHSEE SLHYEGRAVD ITTSDRDRSK YGMLARLAVE AGFDWVYYES181KAHIHCSVKA ENSVAAKSGG CFPGSATVHL EQGGTKLVKD LSPGDRVLAA DDQGRLLYSD241FLTFLDRDDG AKKVFYVIET REPRERLLLT AAHLLFVAPH NDSATGEPEA SSGSGPPSGG301ALGPRALFAS RVRPGQRVYV VAERDGDRRL LPAAVHSVTL SEEAAGAYAP LTAQGTILIN361RVLASCYAVI EEHSWAHRAF APFRLAHALL AALAPARTDR GGDSGGGDRG GGGGRVALTA421PGAADAPGAG ATAGIHWYSQ LLYQIGTWLL DSEALHPLGM AVKSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
465 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.