LSBio
Monoclonal Mouse anti-Human SHH / Sonic Hedgehog Antibody (clone OTI3A2, IF, WB)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.

Monoclonal Mouse anti-Human SHH / Sonic Hedgehog Antibody (clone OTI3A2, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human SHH / Sonic Hedgehog antibodies →
$443.00each
Catalog Number: LS-C114335-100

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Overview

Description
Sonic Hedgehog antibody LS-C114335 is an unconjugated mouse monoclonal antibody to human Sonic Hedgehog (SHH). Validated for IF and WB.
Application
IF, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
HHG-1 | HLP3 | Sonic hedgehog | Sonic hedgehog homolog | MCOPCB5 | SHH | Sonic hedgehog protein | HHG1 | HPE3 | SMMCI | TPT | TPTPS
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: WB
1:2000
UniProt Number
Q15465
NCBI GenBank
NM_000193 | NP_000184.1
SwissProt
Q15465

Target

Target
Human SHH / Sonic Hedgehog
Antigen Species
Human
Epitope
Human Sonic Hedgehog / SHH
Gene Name
SHH

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OTI3A2
Isotype
IgG2a

Immunogen

Immunogen
Recombinant protein expressed in E. coli corresponding to amino acids 24-197 of human SHH.
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Concentration
0.12 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.