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SHB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SHB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117604-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHB (NM_003028), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA3J10.2 | RP11-3J10.8 | SHB
Origin Species
Human
NCBI GenBank
NM_003028
SwissProt
Q15464

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.53 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRAHEGREI PSLGGARRRE VLQAGRSQRA AGRRRRRQEL ELGVGSGRPG GPPPGPGRRG
61TCAAALPPEW PRRRTGLPRR GPRPPLAMAK WLNKYFSLGN SKTKSPPQPP RPDYREQRRR
121GERPSQPPQA VPQASSAASA SCGPATASCF SASSGSLPDD SGSTSDLIRA YRAQKERHFQ
181DPYNGPGSSL RKLRAMCRLD YCGGSGEPGG VQRAFSASSA SGAAGCCCAS SGAGAAASSS
241SSSGSPHLYR SSSERRPATP AEVRYISPKH RLIKVESAAG GGAGDPLGGA CAGGRTWSPT
301ACGGKKLLNK CAASAAEESG AGKKDKVTIA DDYSDPFDAK NDLKSKAGKG ESAGYMEPYE
361AQRIMTEFQR QESVRSQHKG IQLYDTPYEP EGQSVDSDSE STVSPRLRES KLPQDDDRPA
421DEYDQPWEWN RVTSPALAAQ FNGNEKRQSS PSPSRDRRRQ LRAPGGGFKP IKHGSPEFCG
481ILGERVDPAV PLEKQIWYHG AISRGDAENL LRLCKECSYL VRNSQTSKHD YPLSLRSNQG
541FMHMKLAKTK EKYVLGQNSP PFDSVPEVIH YYTTRKLPIK GAEHLSLLYP VAVRTL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

596 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.