SHB Antibody
Catalog Number: LS-C824608-100
100 µg / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SHB Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G78807-20
Catalog Number: LS-G78807
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SHB (NM_003028) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA3J10.2 | RP11-3J10.8 | SHB
- Origin Species
- Human
- NCBI GenBank
- NM_003028
- SwissProt
- Q15464
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.53 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRRAHEGREI PSLGGARRRE VLQAGRSQRA AGRRRRRQEL ELGVGSGRPG GPPPGPGRRG61TCAAALPPEW PRRRTGLPRR GPRPPLAMAK WLNKYFSLGN SKTKSPPQPP RPDYREQRRR121GERPSQPPQA VPQASSAASA SCGPATASCF SASSGSLPDD SGSTSDLIRA YRAQKERHFQ181DPYNGPGSSL RKLRAMCRLD YCGGSGEPGG VQRAFSASSA SGAAGCCCAS SGAGAAASSS241SSSGSPHLYR SSSERRPATP AEVRYISPKH RLIKVESAAG GGAGDPLGGA CAGGRTWSPT301ACGGKKLLNK CAASAAEESG AGKKDKVTIA DDYSDPFDAK NDLKSKAGKG ESAGYMEPYE361AQRIMTEFQR QESVRSQHKG IQLYDTPYEP EGQSVDSDSE STVSPRLRES KLPQDDDRPA421DEYDQPWEWN RVTSPALAAQ FNGNEKRQSS PSPSRDRRRQ LRAPGGGFKP IKHGSPEFCG481ILGERVDPAV PLEKQIWYHG AISRGDAENL LRLCKECSYL VRNSQTSKHD YPLSLRSNQG541FMHMKLAKTK EKYVLGQNSP PFDSVPEVIH YYTTRKLPIK GAEHLSLLYP VAVRTLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
596 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
