Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SHANK2 / SHANK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G129199-100
Catalog Number: LS-G129199
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHANK2 (NM_012309), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AUTS17 | Cortactin binding protein 1 | CTTNBP1 | CORTBP1 | SHANK2 | SPANK-3 | SHANK | Cortactin-binding protein 1 | GKAP/SAPAP interacting protein | KIAA1022 | ProSAP1
- Origin Species
- Human
- NCBI GenBank
- NM_012309
- SwissProt
- Q9UPX8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 201.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRSPTSSED EMAQSFSDYS VGSESDSSKE ETIYDTIRAT AEKPGGARTE ESQGNTLVIR61VVIHDLQQTK CIRFNPDATV WVAKQRILCT LTQSLKDVLN YGLFQPASNG RDGKFLDEER121LLREYPQPVG EGVPSLEFRY KKRVYKQASL DEKQLAKLHT KTNLKKCMDH IQHRLVEKIT181KMLDRGLDPN FHDPETGETP LTLAAQLDDS VEVIKALKNG GAHLDFRAKD GMTALHKAAR241ARNQVALKTL LELGASPDYK DSYGLTPLYH TAIVGGDPYC CELLLHEHAT VCCKDENGWH301EIHQACRYGH VQHLEHLLFY GADMSAQNAS GNTALHICAL YNQDSCARVL LFRGGNKELK361NYNSQTPFQV AIIAGNFELA EYIKNHKETD IVPFREAPAY SNRRRRPPNT LAAPRVLLRS421NSDNNLNASA PDWAVCSTAT SHRSLSPQLL QQMPSKPEGA AKTIGSYVPG PRSRSPSLNR481LGGAGEDGKR PQPLWHVGSP FALGANKDSL SAFEYPGPKR KLYSAVPGRL FVAVKPYQPQ541VDGEIPLHRG DRVKVLSIGE GGFWEGSARG HIGWFPAECV EEVQCKPRDS QAETRADRSK601KLFRHYTVGS YDSFDTSSDC IIEEKTVVLQ KKDNEGFGFV LRGAKADTPI EEFTPTPAFP661ALQYLESVDE GGVAWQAGLR TGDFLIEVNN ENVVKVGHRQ VVNMIRQGGN HLVLKVVTVT721RNLDPDDTAR KKAPPPPKRA PTTALTLRSK SMTSELEELV DKASVRKKKD KPEEIVPASK781PSRAAENMAV EPRVATIKQR PSSRCFPAGS DMNSVYERQG IAVMTPTVPG SPKAPFLGIP841RGTMRRQKSI DSRIFLSGIT EEERQFLAPP MLKFTRSLSM PDTSEDIPPP PQSVPPSPPP901PSPTTYNCPK SPTPRVYGTI KPAFNQNSAA KVSPATRSDT VATMMREKGM YFRRELDRYS961LDSEDLYSRN AGPQANFRNK RGQMPENPYS EVGKIASKAV YVPAKPARRK GMLVKQSNVE1021DSPEKTCSIP IPTIIVKEPS TSSSGKSSQG SSMEIDPQAP EPPSQLRPDE SLTVSSPFAA1081AIAGAVRDRE KRLEARRNSP AFLSTDLGDE DVGLGPPAPR TRPSMFPEEG DFADEDSAEQ1141LSSPMPSATP REPENHFVGG AEASAPGEAG RPLNSTSKAQ GPESSPAVPS ASSGTAGPGN1201YVHPLTGRLL DPSSPLALAL SARDRAMKES QQGPKGEAPK ADLNKPLYID TKMRPSLDAG1261FPTVTRQNTR GPLRRQETEN KYETDLGRDR KGDDKKNMLI DIMDTSQQKS AGLLMVHTVD1321ATKLDNALQE EDEKAEVEMK PDSSPSEVPE GVSETEGALQ ISAAPEPTTV PGRTIVAVGS1381MEEAVILPFR IPPPPLASVD LDEDFIFTEP LPPPLEFANS FDIPDDRAAS VPALSDLVKQ1441KKSDTPQSPS LNSSQPTNSA DSKKPASLSN CLPASFLPPP ESFDAVADSG IEEVDSRSSS1501DHHLETTSTI STVSSISTLS SEGGENVDTC TVYADGQAFM VDKPPVPPKP KMKPIIHKSN1561ALYQDALVEE DVDSFVIPPP APPPPPGSAQ PGMAKVLQPR TSKLWGDVTE IKSPILSGPK1621ANVISELNSI LQQMNREKLA KPGEGLDSPM GAKSASLAPR SPEIMSTISG TRSTTVTFTV1681RPGTSQPITL QSRPPDYESR TSGTRRAPSP VVSPTEMNKE TLPAPLSAAT ASPSPALSDV1741FSLPSQPPSG DLFGLNPAGR SRSPSPSILQ QPISNKPFTT KPVHLWTKPD VADWLESLNL1801GEHKEAFMDN EIDGSHLPNL QKEDLIDLGV TRVGHRMNIE RALKQLLDRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1849 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.