LSBio
Human SHANK2 / SHANK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SHANK2 / SHANK Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SHANK2 antibodies →
$220.00each
Catalog Number: LS-G82802-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SHANK2 (NM_012309) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AUTS17 | Cortactin binding protein 1 | CTTNBP1 | CORTBP1 | SHANK2 | SPANK-3 | SHANK | Cortactin-binding protein 1 | GKAP/SAPAP interacting protein | KIAA1022 | ProSAP1
Origin Species
Human
NCBI GenBank
NM_012309
SwissProt
Q9UPX8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
201.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRSPTSSED EMAQSFSDYS VGSESDSSKE ETIYDTIRAT AEKPGGARTE ESQGNTLVIR
61VVIHDLQQTK CIRFNPDATV WVAKQRILCT LTQSLKDVLN YGLFQPASNG RDGKFLDEER
121LLREYPQPVG EGVPSLEFRY KKRVYKQASL DEKQLAKLHT KTNLKKCMDH IQHRLVEKIT
181KMLDRGLDPN FHDPETGETP LTLAAQLDDS VEVIKALKNG GAHLDFRAKD GMTALHKAAR
241ARNQVALKTL LELGASPDYK DSYGLTPLYH TAIVGGDPYC CELLLHEHAT VCCKDENGWH
301EIHQACRYGH VQHLEHLLFY GADMSAQNAS GNTALHICAL YNQDSCARVL LFRGGNKELK
361NYNSQTPFQV AIIAGNFELA EYIKNHKETD IVPFREAPAY SNRRRRPPNT LAAPRVLLRS
421NSDNNLNASA PDWAVCSTAT SHRSLSPQLL QQMPSKPEGA AKTIGSYVPG PRSRSPSLNR
481LGGAGEDGKR PQPLWHVGSP FALGANKDSL SAFEYPGPKR KLYSAVPGRL FVAVKPYQPQ
541VDGEIPLHRG DRVKVLSIGE GGFWEGSARG HIGWFPAECV EEVQCKPRDS QAETRADRSK
601KLFRHYTVGS YDSFDTSSDC IIEEKTVVLQ KKDNEGFGFV LRGAKADTPI EEFTPTPAFP
661ALQYLESVDE GGVAWQAGLR TGDFLIEVNN ENVVKVGHRQ VVNMIRQGGN HLVLKVVTVT
721RNLDPDDTAR KKAPPPPKRA PTTALTLRSK SMTSELEELV DKASVRKKKD KPEEIVPASK
781PSRAAENMAV EPRVATIKQR PSSRCFPAGS DMNSVYERQG IAVMTPTVPG SPKAPFLGIP
841RGTMRRQKSI DSRIFLSGIT EEERQFLAPP MLKFTRSLSM PDTSEDIPPP PQSVPPSPPP
901PSPTTYNCPK SPTPRVYGTI KPAFNQNSAA KVSPATRSDT VATMMREKGM YFRRELDRYS
961LDSEDLYSRN AGPQANFRNK RGQMPENPYS EVGKIASKAV YVPAKPARRK GMLVKQSNVE
1021DSPEKTCSIP IPTIIVKEPS TSSSGKSSQG SSMEIDPQAP EPPSQLRPDE SLTVSSPFAA
1081AIAGAVRDRE KRLEARRNSP AFLSTDLGDE DVGLGPPAPR TRPSMFPEEG DFADEDSAEQ
1141LSSPMPSATP REPENHFVGG AEASAPGEAG RPLNSTSKAQ GPESSPAVPS ASSGTAGPGN
1201YVHPLTGRLL DPSSPLALAL SARDRAMKES QQGPKGEAPK ADLNKPLYID TKMRPSLDAG
1261FPTVTRQNTR GPLRRQETEN KYETDLGRDR KGDDKKNMLI DIMDTSQQKS AGLLMVHTVD
1321ATKLDNALQE EDEKAEVEMK PDSSPSEVPE GVSETEGALQ ISAAPEPTTV PGRTIVAVGS
1381MEEAVILPFR IPPPPLASVD LDEDFIFTEP LPPPLEFANS FDIPDDRAAS VPALSDLVKQ
1441KKSDTPQSPS LNSSQPTNSA DSKKPASLSN CLPASFLPPP ESFDAVADSG IEEVDSRSSS
1501DHHLETTSTI STVSSISTLS SEGGENVDTC TVYADGQAFM VDKPPVPPKP KMKPIIHKSN
1561ALYQDALVEE DVDSFVIPPP APPPPPGSAQ PGMAKVLQPR TSKLWGDVTE IKSPILSGPK
1621ANVISELNSI LQQMNREKLA KPGEGLDSPM GAKSASLAPR SPEIMSTISG TRSTTVTFTV
1681RPGTSQPITL QSRPPDYESR TSGTRRAPSP VVSPTEMNKE TLPAPLSAAT ASPSPALSDV
1741FSLPSQPPSG DLFGLNPAGR SRSPSPSILQ QPISNKPFTT KPVHLWTKPD VADWLESLNL
1801GEHKEAFMDN EIDGSHLPNL QKEDLIDLGV TRVGHRMNIE RALKQLLDR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1849 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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