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SH3D19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SH3D19 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G129126-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SH3D19 (NM_001009555), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM-binding protein Eve-1 | EEN-binding protein | SH3 domain containing 19 | SH3P19 | EVE1 | Kryn | SH3 domain protein D19 | SH3D19
Origin Species
Human
NCBI GenBank
AL133047
SwissProt
Q5HYK7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNIMNTEQSQ NSIVSRIKVF EGQTNIETSG LPKKPEITPR SLPPKPTVSS GKPSVAPKPA
61ANRASGEWDS GTENRLKVTS KEGLTPYPPL QEAGSIPVTK PELPKKPNPG LIRSVNPEIP
121GRGPLAESSD SGKKVPTPAP RPLLLKKSVS SENPTYPSAP LKPVTVPPRL AGASQAKAYK
181SLGEGPPANP PVPVLQSKPL VDIDLISFDD DVLPTPSGNL AEESVGSEMV LDPFQLPAKT
241EPIKERAVQP APTRKPTVIR IPAKPGKCLH EDPQSPPPLP AEKPIGNTFS TVSGKLSNVE
301RTRNLESNHP GQTGGFVRVP PRLPPRPVNG KTIPTQQPPT KVPPERPPPP KLSATRRSNK
361KLPFNRSSSD MDLQKKQSNL ATGLSKAKSQ VFKNQDPVLP PRPKPGHPLY SKYMLSVPHG
421IANEDIVSQN PGELSCKRGD VLVMLKQTEN NYLECQKGED TGRVHLSQMK IITPLDEHLR
481SRPNDPSHAQ KPVDSGAPHA VVLHDFPAEQ VDDLNLTSGE IVYLLEKIDT DWYRGNCRNQ
541IGIFPANYVK VIIDIPEGGN GKRECVSSHC VKGSRCVARF EYIGEQKDEL SFSEGEIIIL
601KEYVNEEWAR GEVRGRTGIF PLNFVEPVED YPTSGANVLS TKVPLKTKKE DSGSNSQVNS
661LPAEWCEALH SFTAETSDDL SFKRGDRIQI LERLDSDWCR GRLQDREGIF PAVFVRPCPA
721EAKSMLAIVP KGRKAKALYD FRGENEDELS FKAGDIITEL ESVDDDWMSG ELMGKSGIFP
781KNYIQFLQIS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

790 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.