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Human SH3D19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SH3D19 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SH3D19 antibodies →
$220.00each
Catalog Number: LS-G85661-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SH3D19 (NM_001009555) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADAM-binding protein Eve-1 | EEN-binding protein | SH3 domain containing 19 | SH3P19 | EVE1 | Kryn | SH3 domain protein D19 | SH3D19
Origin Species
Human
NCBI GenBank
AL133047
SwissProt
Q5HYK7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNIMNTEQSQ NSIVSRIKVF EGQTNIETSG LPKKPEITPR SLPPKPTVSS GKPSVAPKPA
61ANRASGEWDS GTENRLKVTS KEGLTPYPPL QEAGSIPVTK PELPKKPNPG LIRSVNPEIP
121GRGPLAESSD SGKKVPTPAP RPLLLKKSVS SENPTYPSAP LKPVTVPPRL AGASQAKAYK
181SLGEGPPANP PVPVLQSKPL VDIDLISFDD DVLPTPSGNL AEESVGSEMV LDPFQLPAKT
241EPIKERAVQP APTRKPTVIR IPAKPGKCLH EDPQSPPPLP AEKPIGNTFS TVSGKLSNVE
301RTRNLESNHP GQTGGFVRVP PRLPPRPVNG KTIPTQQPPT KVPPERPPPP KLSATRRSNK
361KLPFNRSSSD MDLQKKQSNL ATGLSKAKSQ VFKNQDPVLP PRPKPGHPLY SKYMLSVPHG
421IANEDIVSQN PGELSCKRGD VLVMLKQTEN NYLECQKGED TGRVHLSQMK IITPLDEHLR
481SRPNDPSHAQ KPVDSGAPHA VVLHDFPAEQ VDDLNLTSGE IVYLLEKIDT DWYRGNCRNQ
541IGIFPANYVK VIIDIPEGGN GKRECVSSHC VKGSRCVARF EYIGEQKDEL SFSEGEIIIL
601KEYVNEEWAR GEVRGRTGIF PLNFVEPVED YPTSGANVLS TKVPLKTKKE DSGSNSQVNS
661LPAEWCEALH SFTAETSDDL SFKRGDRIQI LERLDSDWCR GRLQDREGIF PAVFVRPCPA
721EAKSMLAIVP KGRKAKALYD FRGENEDELS FKAGDIITEL ESVDDDWMSG ELMGKSGIFP
781KNYIQFLQIS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

790 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.