Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SH2D3C / NSP3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115937-100
Catalog Number: LS-G115937
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NSP3(SH2D3C) (NM_005489), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Novel SH2-containing protein 3 | SH2D3C | PRO34088 | SH2 domain-containing 3C | NSP3 | SH2 domain containing 3C
- Origin Species
- Human
- NCBI GenBank
- NM_005489
- SwissProt
- Q8N5H7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAVGRRCPA LGSRGAAGEP EAGSDYVKFS KEKYILDSSP EKLHKELEEE LKLSSTDLRS61HAWYHGRIPR EVSETLVQRN GDFLIRDSLT SLGDYVLTCR WRNQALHFKI NKVVVKAGES121YTHIQYLFEQ ESFDHVPALV RYHVGSRKAV SEQSGAIIYC PVNRTFPLRY LEASYGLGQG181SSKPASPVSP SGPKGSHMKR RSVTMTDGLT ADKVTRSDGC PTSTSLPRPR DSIRSCALSM241DQIPDLHSPM SPISESPSSP AYSTVTRVHA APAAPSATAL PASPVARRSS EPQLCPGSAP301KTHGESDKGP HTSPSHTLGK ASPSPSLSSY SDPDSGHYCQ LQPPVRGSRE WAATETSSQQ361ARSYGERLKE LSENGAPEGD WGKTFTVPIV EVTSSFNPAT FQSLLIPRDN RPLEVGLLRK421VKELLAEVDA RTLARHVTKV DCLVARILGV TKEMQTLMGV RWGMELLTLP HGRQLRLDLL481ERFHTMSIML AVDILGCTGS AEERAALLHK TIQLAAELRG TMGNMFSFAA VMGALDMAQI541SRLEQTWVTL RQRHTEGAIL YEKKLKPFLK SLNEGKEGPP LSNTTFPHVL PLITLLECDS601APPEGPEPWG STEHGVEVVL AHLEAARTVA HHGGLYHTNA EVKLQGFQAR PELLEVFSTE661FQMRLLWGSQ GASSSQARRY EKFDKVLTAL SHKLEPAVRS SELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
703 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.