Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SH2D3C / NSP3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105641-100
Catalog Number: LS-G105641
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NSP3(SH2D3C) (NM_170600), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Novel SH2-containing protein 3 | SH2D3C | PRO34088 | SH2 domain-containing 3C | NSP3 | SH2 domain containing 3C
- Origin Species
- Human
- NCBI GenBank
- NM_005489
- SwissProt
- Q8N5H7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 94.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTEGTKKTSK KFKFFKFKGF GSLSNLPRSF TLRRSSASIS RQSHLEPDTF EATQDDMVTV61PKSPPAYARS SDMYSHMGTM PRPSIKKAQN SQAARQAQEA GPKPNLVPGG VPDPPGLEAA121KEVMVKATGP LEDTPAMEPN PSAVEVDPIR KPEVPTGDVE EERPPRDVHS ERAAGEPEAG181SDYVKFSKEK YILDSSPEKL HKELEEELKL SSTDLRSHAW YHGRIPREVS ETLVQRNGDF241LIRDSLTSLG DYVLTCRWRN QALHFKINKV VVKAGESYTH IQYLFEQESF DHVPALVRYH301VGSRKAVSEQ SGAIIYCPVN RTFPLRYLEA SYGLGQGSSK PASPVSPSGP KGSHMKRRSV361TMTDGLTADK VTRSDGCPTS TSLPRPRDSI RSCALSMDQI PDLHSPMSPI SESPSSPAYS421TVTRVHAAPA APSATALPAS PVARRSSEPQ LCPGSAPKTH GESDKGPHTS PSHTLGKASP481SPSLSSYSDP DSGHYCQLQP PVRGSREWAA TETSSQQARS YGERLKELSE NGAPEGDWGK541TFTVPIVEVT SSFNPATFQS LLIPRDNRPL EVGLLRKVKE LLAEVDARTL ARHVTKVDCL601VARILGVTKE MQTLMGVRWG MELLTLPHGR QLRLDLLERF HTMSIMLAVD ILGCTGSAEE661RAALLHKTIQ LAAELRGTMG NMFSFAAVMG ALDMAQISRL EQTWVTLRQR HTEGAILYEK721KLKPFLKSLN EGKEGPPLSN TTFPHVLPLI TLLECDSAPP EGPEPWGSTE HGVEVVLAHL781EAARTVAHHG GLYHTNAEVK LQGFQARPEL LEVFSTEFQM RLLWGSQGAS SSQARRYEKF841DKVLTALSHK LEPAVRSSEQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
860 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.