Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SH2B2 / APS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G129087-100
Catalog Number: LS-G129087
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SH2B2 (NM_020979), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APS | SH2B adapter protein 2 | SH2B adaptor protein 2 | SH2B2
- Origin Species
- Human
- NCBI GenBank
- NM_020979
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPSYCPAHG FPSQDPLWPL SSQQWSSAHY SEPAAGGCDG TEAMNGAGPG PAAAAPVPVP61VPVPDWRQFC ELHAQAAAVD FAHKFCRFLR DNPAYDTPDA GASFSRHFAA NFLDVFGEEV121RRVLVAGPTT RGAAVSAEAM EPELADTSAL KAAPYGHSRS SEDVSTHAAT KARVRKGFSL181RNMSLCVVDG VRDMWHRRAS PEPDAAAAPR TAEPRDKWTR RLRLSRTLAA KVELVDIQRE241GALRFMVADD AAAGSGGSAQ WQKCRLLLRR AVAEERFRLE FFVPPKASRP KVSIPLSAII301EVRTTMPLEM PEKDNTFVLK VENGAEYILE TIDSLQKHSW VADIQGCVDP GDSEEDTELS361CTRGGCLASR VASCSCELLT DAVDLPRPPE TTAVGAVVTA PHSRGRDAVR ESLIHVPLET421FLQTLESPGG SGSDSNNTGE QGAETDPEAE PELELSDYPW FHGTLSRVKA AQLVLAGGPR481NHGLFVIRQS ETRPGEYVLT FNFQGKAKHL RLSLNGHGQC HVQHLWFQSV LDMLRHFHTH541PIPLESGGSA DITLRSYVRA QDPPPEPGPT PPAAPASPAC WSDSPGQHYF SSLAAAACPP601ASPSDAAGAS SSSASSSSAA SGPAPPRPVE GQLSARSRSN SAERLLEAVA ATAAEEPPEA661APGRARAVEN QYSFYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
675 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.