SH2B2 / APS Antibody
Catalog Number: LS-C805414
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SH2B2 / APS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G79703-20
Catalog Number: LS-G79703
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SH2B2 (NM_020979) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APS | SH2B adapter protein 2 | SH2B adaptor protein 2 | SH2B2
- Origin Species
- Human
- NCBI GenBank
- NM_020979
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPSYCPAHG FPSQDPLWPL SSQQWSSAHY SEPAAGGCDG TEAMNGAGPG PAAAAPVPVP61VPVPDWRQFC ELHAQAAAVD FAHKFCRFLR DNPAYDTPDA GASFSRHFAA NFLDVFGEEV121RRVLVAGPTT RGAAVSAEAM EPELADTSAL KAAPYGHSRS SEDVSTHAAT KARVRKGFSL181RNMSLCVVDG VRDMWHRRAS PEPDAAAAPR TAEPRDKWTR RLRLSRTLAA KVELVDIQRE241GALRFMVADD AAAGSGGSAQ WQKCRLLLRR AVAEERFRLE FFVPPKASRP KVSIPLSAII301EVRTTMPLEM PEKDNTFVLK VENGAEYILE TIDSLQKHSW VADIQGCVDP GDSEEDTELS361CTRGGCLASR VASCSCELLT DAVDLPRPPE TTAVGAVVTA PHSRGRDAVR ESLIHVPLET421FLQTLESPGG SGSDSNNTGE QGAETDPEAE PELELSDYPW FHGTLSRVKA AQLVLAGGPR481NHGLFVIRQS ETRPGEYVLT FNFQGKAKHL RLSLNGHGQC HVQHLWFQSV LDMLRHFHTH541PIPLESGGSA DITLRSYVRA QDPPPEPGPT PPAAPASPAC WSDSPGQHYF SSLAAAACPP601ASPSDAAGAS SSSASSSSAA SGPAPPRPVE GQLSARSRSN SAERLLEAVA ATAAEEPPEA661APGRARAVEN QYSFYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
675 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
