Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SGMS1 / TMEM23 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106539-100
Catalog Number: LS-G106539
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sphingomyelin Synthase 1(SGMS1) (NM_147156), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hmob33 | MOB | MOB1 | Sphingomyelin synthase 1 | TMEM23 | Transmembrane protein 23 | Protein Mob | SMS1 | SGMS1
- Origin Species
- Human
- NCBI GenBank
- NM_147156
- SwissProt
- Q86VZ5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKEVVYWSPK KVADWLLENA MPEYCEPLEH FTGQDLINLT QEDFKKPPLC RVSSDNGQRL61LDMIETLKME HHLEAHKNGH ANGHLNIGVD IPTPDGSFSI KIKPNGMPNG YRKEMIKIPM121PELERSQYPM EWGKTFLAFL YALSCFVLTT VMISVVHERV PPKEVQPPLP DTFFDHFNRV181QWAFSICEIN GMILVGLWLI QWLLLKYKSI ISRRFFCIVG TLYLYRCITM YVTTLPVPGM241HFNCSPKLFG DWEAQLRRIM KLIAGGGLSI TGSHNMCGDY LYSGHTVMLT LTYLFIKEYS301PRRLWWYHWI CWLLSVVGIF CILLAHDHYT VDVVVAYYIT TRLFWWYHTM ANQQVLKEAS361QMNLLARVWW YRPFQYFEKN VQGIVPRSYH WPFPWPVVHL SRQVKYSRLV NDTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
413 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.