LSBio
Human SGMS1 / TMEM23 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SGMS1 / TMEM23 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G82434-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Sphingomyelin Synthase 1(SGMS1) (NM_147156) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Hmob33 | MOB | MOB1 | Sphingomyelin synthase 1 | TMEM23 | Transmembrane protein 23 | Protein Mob | SMS1 | SGMS1
Origin Species
Human
NCBI GenBank
NM_147156
SwissProt
Q86VZ5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKEVVYWSPK KVADWLLENA MPEYCEPLEH FTGQDLINLT QEDFKKPPLC RVSSDNGQRL
61LDMIETLKME HHLEAHKNGH ANGHLNIGVD IPTPDGSFSI KIKPNGMPNG YRKEMIKIPM
121PELERSQYPM EWGKTFLAFL YALSCFVLTT VMISVVHERV PPKEVQPPLP DTFFDHFNRV
181QWAFSICEIN GMILVGLWLI QWLLLKYKSI ISRRFFCIVG TLYLYRCITM YVTTLPVPGM
241HFNCSPKLFG DWEAQLRRIM KLIAGGGLSI TGSHNMCGDY LYSGHTVMLT LTYLFIKEYS
301PRRLWWYHWI CWLLSVVGIF CILLAHDHYT VDVVVAYYIT TRLFWWYHTM ANQQVLKEAS
361QMNLLARVWW YRPFQYFEKN VQGIVPRSYH WPFPWPVVHL SRQVKYSRLV NDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

413 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.