Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SFTPD / Surfactant Protein D (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99940-100
Catalog Number: LS-G99940
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Surfactant protein D(SFTPD) (NM_003019), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COLEC7 | Collectin 7 | Collectin-7 | Lung surfactant protein D | PSP-D | PSPD | SP-D | SFTPD | SFTP4 | Surfactant protein D
- NCBI GenBank
- NM_003019
- SwissProt
- P35247
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.66 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLFLLSALV LLTQPLGYLE AGMKTYSHRT MPSACTLVMC SSVESGLPGR DGRDGREGPR61GEKGDPGLPG AAGQAGMPGQ AGPVGPKGDN GSVGEPGPKG DTGPSGPPGP PGVPGPAGRE121GPLGKQGNIG PQGKPGPKGE AGPKGEVGAP GMQGSAGARG LAGPKGERGV PGERGVPGNT181GAAGSAGAMG PQGSPGARGP PGLKGDKGIP GDKGAKGESG LPDVASLRQQ VEALQGQVQH241LQAAFSQYKK VELFPNGQSV GEKIFKTAGF VKPFTEAQLL CTQAGGQLAS PRSAAENAAL301QQLVVAKNEA AFLSMTDSKT EGKFTYPTGE SLVYSNWAPG KPNDDGGSED CVEIFTNGKW361NDRACGEKRL VVCEFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
375 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.