Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SERPINE1 / PAI-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128094-100
Catalog Number: LS-G128094
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAI1(SERPINE1) (NM_001165413), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PAI1 | PLANH1 | SERPINE1 | PAI-1 | Serpin E1
- Origin Species
- Human
- NCBI GenBank
- NM_000602
- SwissProt
- P05121
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQMSPALTCL VLGLALVFGE GSAVHHPPSY VAQASKDRNV VFSPYGVASV LAMLQLTTGG61ETQQQIQAAM GFKIDDKGMA PALRHLYKEL MGPWNKDEIS TTDAIFVQRD LKLVQGFMPH121FFRLFRSTVK QVDFSEVERA RFIINDWVKT HTKGMISNLL GKGAVDQLTR LVLVNALYFN181GQWKTPFPDS STHRRLFHKS DGSTVSVPMM AQTNKFNYTE FTTPDGHYYD ILELPYHGDT241LSMFIAAPYE KEVPLSALTN ILSAQLISHW KGNMTRLPRL LVLPKFSLET EVDLRKPLEN301LGMTDMFRQF QADFTSLSDQ EPLHVAQALQ KVKIEVNESG TVASSSTAVI VSARMAPEEI361IMDRPFLFVV RHNPTGTVLF MGQVMEPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
387 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.