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SEMG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SEMG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117618-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Semenogelin II(SEMG2) (NM_003008), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Semenogelin II | Semenogelin 2 | Semenogelin-2 | SEMG2 | SGII
Origin Species
Human
NCBI GenBank
NM_003008
SwissProt
Q02383

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.47 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKSIILFVLS LLLILEKQAA VMGQKGGSKG QLPSGSSQFP HGKKGQHYFG QKDQQHTKSK
61GSFSIQHTYH VDINDHDWTR KSQQYDLNAL HKATKSKQHL GGSQQLLNYK QEGRDHDKSK
121GHFHMIVIHH KGGQAHHGTQ NPSQDQGNSP SGKGLSSQCS NTEKRLWVHG LSKEQASASG
181AQKGRTQGGS QSSYVLQTEE LVVNKQQRET KNSHQNKGHY QNVVDVREEH SSKLQTSLHP
241AHQDRLQHGP KDIFTTQDEL LVYNKNQHQT KNLNQDQEHG RKAHKISYPS SRTEERQLHH
301GEKSVQKDVS KGSISIQTEE KIHGKSQNQV TIHSQDQEHG HKENKISYQS SSTEERHLNC
361GEKGIQKGVS KGSISIQTEE QIHGKSQNQV RIPSQAQEYG HKENKISYQS SSTEERRLNS
421GEKDVQKGVS KGSISIQTEE KIHGKSQNQV TIPSQDQEHG HKENKMSYQS SSTEERRLNY
481GGKSTQKDVS QSSISFQIEK LVEGKSQIQT PNPNQDQWSG QNAKGKSGQS ADSKQDLLSH
541EQKGRYKQES SESHNIVITE HEVAQDDHLT QQYNEDRNPI ST
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

582 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.