Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA4B / Semaphorin 4B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111277-100
Catalog Number: LS-G111277
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEMA4B (NM_020210), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1745 | SEMAC | Semaphorin-4B | SemC | SEMA4B
- Origin Species
- Human
- NCBI GenBank
- AK026108
- SwissProt
- Q9NPR2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLRTAMGLRS WLAAPWGALP PRPPLLLLLL LLLLLQPPPP TWALSPRISL PLGSEERPFL61RFEAEHISNY TALLLSRDGR TLYVGAREAL FALSSNLSFL PGGEYQELLW GADAEKKQQC121SFKGKDPQRD CQNYIKILLP LSGSHLFTCG TAAFSPMCTY INMENFTLAR DEKGNVLLED181GKGRCPFDPN FKSTALVVDG ELYTGTVSSF QGNDPAISRS QSLRPTKTES SLNWLQDPAF241VASAYIPESL GSLQGDDDKI YFFFSETGQE FEFFENTIVS RIARICKGDE GGERVLQQRW301TSFLKAQLLC SRPDDGFPFN VLQDVFTLSP SPQDWRDTLF YGVFTSQWHR GTTEGSAVCV361FTMKDVQRVF SGLYKEVNRE TQQWYTVTHP VPTPRPGACI TNSARERKIN SSLQLPDRVL421NFLKDHFLMD GQVRSRMLLL QPQARYQRVA VHRVPGLHHT YDVLFLGTGD GRLHKAVSVG481PRVHIIEELQ IFSSGQPVQN LLLDTHRGLL YAASHSGVVQ VPMANCSLYR SCGDCLLARD541PYCAWSGSSC KHVSLYQPQL ATRPWIQDIE GASAKDLCSA SSVVSPSFVP TGEKPCEQVQ601FQPNTVNTLA CPLLSNLATR LWLRNGAPVN ASASCHVLPT GDLLLVGTQQ LGEFQCWSLE661EGFQQLVASY CPEVVEDGVA DQTDEGGSVP VIISTSRVSA PAGGKASWGA DRSYWKEFLV721MCTLFVLAVL LPVLFLLYRH RNSMKVFLKQ GECASVHPKT CPVVLPPETR PLNGLGPPST781PLDHRGYQSL SDSPPGARVF TESEKRPLSI QDSFVEVSPV CPRPRVRLGS EIRDSVVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
837 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.