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Human SEMA4B / Semaphorin 4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SEMA4B / Semaphorin 4B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SEMA4B antibodies →
$153.00each
Catalog Number: LS-G82409-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SEMA4B (NM_198925) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1745 | SEMAC | Semaphorin-4B | SemC | SEMA4B
Origin Species
Human
NCBI GenBank
AK026108
SwissProt
Q9NPR2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLRTAMGLRS WLAAPWGALP PRPPLLLLLL LLLLLQPPPP TWALSPRISL PLGSEERPFL
61RFEAEHISNY TALLLSRDGR TLYVGAREAL FALSSNLSFL PGGEYQELLW GADAEKKQQC
121SFKGKDPQRD CQNYIKILLP LSGSHLFTCG TAAFSPMCTY INMENFTLAR DEKGNVLLED
181GKGRCPFDPN FKSTALVVDG ELYTGTVSSF QGNDPAISRS QSLRPTKTES SLNWLQDPAF
241VASAYIPESL GSLQGDDDKI YFFFSETGQE FEFFENTIVS RIARICKGDE GGERVLQQRW
301TSFLKAQLLC SRPDDGFPFN VLQDVFTLSP SPQDWRDTLF YGVFTSQWHR GTTEGSAVCV
361FTMKDVQRVF SGLYKEVNRE TQQWYTVTHP VPTPRPGACI TNSARERKIN SSLQLPDRVL
421NFLKDHFLMD GQVRSRMLLL QPQARYQRVA VHRVPGLHHT YDVLFLGTGD GRLHKAVSVG
481PRVHIIEELQ IFSSGQPVQN LLLDTHRGLL YAASHSGVVQ VPMANCSLYR SCGDCLLARD
541PYCAWSGSSC KHVSLYQPQL ATRPWIQDIE GASAKDLCSA SSVVSPSFVP TGEKPCEQVQ
601FQPNTVNTLA CPLLSNLATR LWLRNGAPVN ASASCHVLPT GDLLLVGTQQ LGEFQCWSLE
661EGFQQLVASY CPEVVEDGVA DQTDEGGSVP VIISTSRVSA PAGGKASWGA DRSYWKEFLV
721MCTLFVLAVL LPVLFLLYRH RNSMKVFLKQ GECASVHPKT CPVVLPPETR PLNGLGPPST
781PLDHRGYQSL SDSPPGARVF TESEKRPLSI QDSFVEVSPV CPRPRVRLGS EIRDSVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

837 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.