Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA3G / Semaphorin 3G Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111309-100
Catalog Number: LS-G111309
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEMA3G (NM_020163), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Sem2 | SEMA3G | Semaphorin sem2 | Semaphorin-3G
- Origin Species
- Human
- NCBI GenBank
- NM_020163
- SwissProt
- Q9NS98
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPSAWAICW LLGGLLLHGG SSGPSPGPSV PRLRLSYRDL LSANRSAIFL GPQGSLNLQA61MYLDEYRDRL FLGGLDALYS LRLDQAWPDP REVLWPPQPG QREECVRKGR DPLTECANFV121RVLQPHNRTH LLACGTGAFQ PTCALITVGH RGEHVLHLEP GSVESGRGRC PHEPSRPFAS181TFIDGELYTG LTADFLGREA MIFRSGGPRP ALRSDSDQSL LHDPRFVMAA RIPENSDQDN241DKVYFFFSET VPSPDGGSNH VTVSRVGRVC VNDAGGQRVL VNKWSTFLKA RLVCSVPGPG301GAETHFDQLE DVFLLWPKAG KSLEVYALFS TVSAVFQGFA VCVYHMADIW EVFNGPFAHR361DGPQHQWGPY GGKVPFPRPG VCPSKMTAQP GRPFGSTKDY PDEVLQFARA HPLMFWPVRP421RHGRPVLVKT HLAQQLHQIV VDRVEAEDGT YDVIFLGTDS GSVLKVIALQ AGGSAEPEEV481VLEELQVFKV PTPITEMEIS VKRQMLYVGS RLGVAQLRLH QCETYGTACA ECCLARDPYC541AWDGASCTHY RPSLGKRRFR RQDIRHGNPA LQCLGQSQEE EAVGLVAATM VYGTEHNSTF601LECLPKSPQA AVRWLLQRPG DEGPDQVKTD ERVLHTERGL LFRRLSRFDA GTYTCTTLEH661GFSQTVVRLA LVVIVASQLD NLFPPEPKPE EPPARGGLAS TPPKAWYKDI LQLIGFANLP721RVDEYCERVW CRGTTECSGC FRSRSRGKQA RGKSWAGLEL GKKMKSRVHA EHNRTPREVE781ATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
782 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.