SEMA3G / Semaphorin 3G Antibody (aa216-244)
Catalog Number: LS-C168953-400
400 µL (0.44 mg/mL) / $401.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SEMA3G / Semaphorin 3G Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83753-20
Catalog Number: LS-G83753
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SEMA3G (NM_020163) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Sem2 | SEMA3G | Semaphorin sem2 | Semaphorin-3G
- Origin Species
- Human
- NCBI GenBank
- NM_020163
- SwissProt
- Q9NS98
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPSAWAICW LLGGLLLHGG SSGPSPGPSV PRLRLSYRDL LSANRSAIFL GPQGSLNLQA61MYLDEYRDRL FLGGLDALYS LRLDQAWPDP REVLWPPQPG QREECVRKGR DPLTECANFV121RVLQPHNRTH LLACGTGAFQ PTCALITVGH RGEHVLHLEP GSVESGRGRC PHEPSRPFAS181TFIDGELYTG LTADFLGREA MIFRSGGPRP ALRSDSDQSL LHDPRFVMAA RIPENSDQDN241DKVYFFFSET VPSPDGGSNH VTVSRVGRVC VNDAGGQRVL VNKWSTFLKA RLVCSVPGPG301GAETHFDQLE DVFLLWPKAG KSLEVYALFS TVSAVFQGFA VCVYHMADIW EVFNGPFAHR361DGPQHQWGPY GGKVPFPRPG VCPSKMTAQP GRPFGSTKDY PDEVLQFARA HPLMFWPVRP421RHGRPVLVKT HLAQQLHQIV VDRVEAEDGT YDVIFLGTDS GSVLKVIALQ AGGSAEPEEV481VLEELQVFKV PTPITEMEIS VKRQMLYVGS RLGVAQLRLH QCETYGTACA ECCLARDPYC541AWDGASCTHY RPSLGKRRFR RQDIRHGNPA LQCLGQSQEE EAVGLVAATM VYGTEHNSTF601LECLPKSPQA AVRWLLQRPG DEGPDQVKTD ERVLHTERGL LFRRLSRFDA GTYTCTTLEH661GFSQTVVRLA LVVIVASQLD NLFPPEPKPE EPPARGGLAS TPPKAWYKDI LQLIGFANLP721RVDEYCERVW CRGTTECSGC FRSRSRGKQA RGKSWAGLEL GKKMKSRVHA EHNRTPREVE781ATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
782 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
