Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA3B / SemA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122167-100
Catalog Number: LS-G122167
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Semaphorin 3B(SEMA3B) (NM_001005914), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SEMA | SEMA5 | SEMAA | SemaV | Semaphorin A | LuCa-1 | Sema A(V) | Sema V | SEMA3B | Semaphorin-3B | Semaphorin-V
- Origin Species
- Human
- NCBI GenBank
- NM_004636
- SwissProt
- Q13214
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83.03 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRAGAAAVI PGLALLWAVG LGSAAPSPPR LRLSFQELQA WHGLQTFSLE RTCCYQALLV61DEERGRLFVG AENHVASLNL DNISKRAKKL AWPAPVEWRE ECNWAGKDIG TECMNFVKLL121HAYNRTHLLA CGTGAFHPTC AFVEVGHRAE EPVLRLDPGR IEDGKGKSPY DPRHRAASVL181VGEELYSGVA ADLMGRDFTI FRSLGQRPSL RTEPHDSRWL NEPKFVKVFW IPESENPDDD241KIYFFFRETA VEAAPALGRL SVSRVGQICR NDVGGQRSLV NKWTTFLKAR LVCSVPGVEG301DTHFDQLQDV FLLSSRDHRT PLLYAVFSTS SIFQGSAVCV YSMNDVRRAF LGPFAHKEGP361MHQWVSYQGR VPYPRPGMCP SKTFGTFSST KDFPDDVIQF ARNHPLMYNS VLPTGGRPLF421LQVGANYTFT QIAADRVAAA DGHYDVLFIG TDVGTVLKVI SVPKGSRPSA EGLLLEELHV481FEDSAAVTSM QISSKRHQLY VASRSAVAQI ALHRCAAHGR VCTECCLARD PYCAWDGVAC541TRFQPSAKRR FRRQDVRNGD PSTLCSGDSS RPALLEHKVF GVEGSSAFLE CEPRSLQARV601EWTFQRAGVT AHTQVLAEER TERTARGLLL RRLRRRDSGV YLCAAVEQGF TQPLRRLSLH661VLSATQAERL ARAEEAAPAA PPGPKLWYRD FLQLVEPGGG GSANSLRMCR PQPALQSLPL721ESRRKGRNRR THAPEPRAER GPRSATHWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
748 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.