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Human SEMA3B / SemA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SEMA3B / SemA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SEMA3B antibodies →
$153.00each
Catalog Number: LS-G81498-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Semaphorin 3B(SEMA3B) (NM_001005914) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SEMA | SEMA5 | SEMAA | SemaV | Semaphorin A | LuCa-1 | Sema A(V) | Sema V | SEMA3B | Semaphorin-3B | Semaphorin-V
Origin Species
Human
NCBI GenBank
NM_004636
SwissProt
Q13214

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.03 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRAGAAAVI PGLALLWAVG LGSAAPSPPR LRLSFQELQA WHGLQTFSLE RTCCYQALLV
61DEERGRLFVG AENHVASLNL DNISKRAKKL AWPAPVEWRE ECNWAGKDIG TECMNFVKLL
121HAYNRTHLLA CGTGAFHPTC AFVEVGHRAE EPVLRLDPGR IEDGKGKSPY DPRHRAASVL
181VGEELYSGVA ADLMGRDFTI FRSLGQRPSL RTEPHDSRWL NEPKFVKVFW IPESENPDDD
241KIYFFFRETA VEAAPALGRL SVSRVGQICR NDVGGQRSLV NKWTTFLKAR LVCSVPGVEG
301DTHFDQLQDV FLLSSRDHRT PLLYAVFSTS SIFQGSAVCV YSMNDVRRAF LGPFAHKEGP
361MHQWVSYQGR VPYPRPGMCP SKTFGTFSST KDFPDDVIQF ARNHPLMYNS VLPTGGRPLF
421LQVGANYTFT QIAADRVAAA DGHYDVLFIG TDVGTVLKVI SVPKGSRPSA EGLLLEELHV
481FEDSAAVTSM QISSKRHQLY VASRSAVAQI ALHRCAAHGR VCTECCLARD PYCAWDGVAC
541TRFQPSAKRR FRRQDVRNGD PSTLCSGDSS RPALLEHKVF GVEGSSAFLE CEPRSLQARV
601EWTFQRAGVT AHTQVLAEER TERTARGLLL RRLRRRDSGV YLCAAVEQGF TQPLRRLSLH
661VLSATQAERL ARAEEAAPAA PPGPKLWYRD FLQLVEPGGG GSANSLRMCR PQPALQSLPL
721ESRRKGRNRR THAPEPRAER GPRSATHW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

748 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.