Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SDE2 / C1orf55 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106139-100
Catalog Number: LS-G106139
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf55(SDE2) (NM_152608), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ671D7.1 | SDE2 | UPF0667 protein C1orf55 | C1orf55
- Origin Species
- Human
- SwissProt
- Q6IQ49
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEAAALVWI RGPGFGCKAV RCASGRCTVR DFIHRHCQDQ NVPVENFFVK CNGALINTSD61TVQHGAVYSL EPRLCGGKGG FGSMLRALGA QIEKTTNREA CRDLSGRRLR DVNHEKAMAE121WVKQQAEREA EKEQKRLERL QRKLVEPKHC FTSPDYQQQC HEMAERLEDS VLKGMQAASS181KMVSAEISEN RKRQWPTKSQ TDRGASAGKR RCFWLGMEGL ETAEGSNSES SDDDSEEAPS241TSGMGFHAPK IGSNGVEMAA KFPSGSQRAR VVNTDHGSPE QLQIPVTDSG RHILEDSCAE301LGESKEHMES RMVTETEETQ EKKAESKEPI EEEPTGAGLN KDKETEERTD GERVAEVAPE361ERENVAVAKL QESQPGNAVI DKETIDLLAF TSVAELELLG LEKLKCELMA LGLKCGGTLQ421ERAARLFSVR GLAKEQIDPA LFAKPLKGKK KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
451 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.