LSBio
Human SDE2 / C1orf55 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SDE2 / C1orf55 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G93313-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C1orf55(SDE2) (NM_152608) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ671D7.1 | SDE2 | UPF0667 protein C1orf55 | C1orf55
Origin Species
Human
SwissProt
Q6IQ49

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAAALVWI RGPGFGCKAV RCASGRCTVR DFIHRHCQDQ NVPVENFFVK CNGALINTSD
61TVQHGAVYSL EPRLCGGKGG FGSMLRALGA QIEKTTNREA CRDLSGRRLR DVNHEKAMAE
121WVKQQAEREA EKEQKRLERL QRKLVEPKHC FTSPDYQQQC HEMAERLEDS VLKGMQAASS
181KMVSAEISEN RKRQWPTKSQ TDRGASAGKR RCFWLGMEGL ETAEGSNSES SDDDSEEAPS
241TSGMGFHAPK IGSNGVEMAA KFPSGSQRAR VVNTDHGSPE QLQIPVTDSG RHILEDSCAE
301LGESKEHMES RMVTETEETQ EKKAESKEPI EEEPTGAGLN KDKETEERTD GERVAEVAPE
361ERENVAVAKL QESQPGNAVI DKETIDLLAF TSVAELELLG LEKLKCELMA LGLKCGGTLQ
421ERAARLFSVR GLAKEQIDPA LFAKPLKGKK K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

451 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.