Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCTR / SR / Secretin Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117628-100
Catalog Number: LS-G117628
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCTR (NM_002980), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Secretin receptor | SR | SCT-R | SCTR
- Origin Species
- Human
- NCBI GenBank
- NM_002980
- SwissProt
- P47872
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.22 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRPHLSPPLQ QLLLPVLLAC AAHSTGALPR LCDVLQVLWE EQDQCLQELS REQTGDLGTE61QPVPGCEGMW DNISCWPSSV PGRMVEVECP RFLRMLTSRN GSLFRNCTQD GWSETFPRPN121LACGVNVNDS SNEKRHSYLL KLKVMYTVGY SSSLVMLLVA LGILCAFRRL HCTRNYIHMH181LFVSFILRAL SNFIKDAVLF SSDDVTYCDA HRAGCKLIMV LFQYCIMANY SWLLVEGLYL241HTLLAISFFS ERKYLQGFVA FGWGSPAIFV ALWAIARHFL EDVGCWDINA NASIWWIIRG301PVILSILINF ILFINILRIL MRKLRTQETR GNEVSHYKRL ARSTLLLIPL FGIHYIVFAF361SPEDAMEIQL FFELALGSFQ GLVVAVLYCF LNGEVQLEVQ KKWQQWHLRE FPLHPVASFS421NSTKASHLEQ SQGTCRTSIITRTRPLEQKLISEEDLAANDILDYKDDDDKV
440 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.