Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCOP / PHLPP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103899-100
Catalog Number: LS-G103899
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHLPP(PHLPP1) (NM_194449), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSCOP | KIAA0606 | PHLPP1 | PLEKHE1 | SCOP | PHLPP
- Origin Species
- Human
- NCBI GenBank
- AB011178
- SwissProt
- O60346
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 184.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSEIGCLIR FYAGKPHSTG SSERIQLSGM YNVRKGKMQL PVNRWTRRQV ILCGTCLIVS61SVKDSLTGKM HVLPLIGGKV EEVKKHQHCL AFSSSGPQSQ TYYICFDTFT EYLRWLRQVS121KVASQRISSV DLSCCSLEHL PANLFYSQDL THLNLKQNFL RQNPSLPAAR GLNELQRFTK181LKSLNLSNNH LGDFPLAVCS IPTLAELNVS CNALRSVPAA VGVMHNLQTF LLDGNFLQSL241PAELENMKQL SYLGLSFNEF TDIPEVLEKL TAVDKLCMSG NCVETLRLQA LRKMPHIKHV301DLRLNVIRKL IADEVDFLQH VTQLDLRDNK LGDLDAMIFN NIEVLHCERN QLVTLDICGY361FLKALYASSN ELVQLDVYPV PNYLSYMDVS RNRLENVPEW VCESRKLEVL DIGHNQICEL421PARLFCNSSL RKLLAGHNQL ARLPERLERT SVEVLDVQHN QLLELPPNLL MKADSLRFLN481ASANKLESLP PATLSEETNS ILQELYLTNN SLTDKCVPLL TGHPHLKILH MAYNRLQSFP541ASKMAKLEEL EEIDLSGNKL KAIPTTIMNC RRMHTVIAHS NCIEVFPEVM QLPEIKCVDL601SCNELSEVTL PENLPPKLQE LDLTGNPRLV LDHKTLELLN NIRCFKIDQP STGDASGAPA661VWSHGYTEAS GVKNKLCVAA LSVNNFCDNR EALYGVFDGD RNVEVPYLLQ CTMSDILAEE721LQKTKNEEEY MVNTFIVMQR KLGTAGQKLG GAAVLCHIKH DPVDPGGSFT LTSANVGKCQ781TVLCRNGKPL PLSRSYIMSC EEELKRIKQH KAIITEDGKV NGVTESTRIL GYTFLHPSVV841PRPHVQSVLL TPQDEFFILG SKGLWDSLSV EEAVEAVRNV PDALAAAKKL CTLAQSYGCH901DSISAVVVQL SVTEDSFCCC ELSAGGAVPP PSPGIFPPSV NMVIKDRPSD GLGVPSSSSG961MASEISSELS TSEMSSEVGS TASDEPPPGA LSENSPAYPS EQRCMLHPIC LSNSFQRQLS1021SATFSSAFSD NGLDSDDEEP IEGVFTNGSR VEVEVDIHCS RAKEKEKQQH LLQVPAEASD1081EGIVISANED EPGLPRKADF SAVGTIGRRR ANGSVAPQER SHNVIEVATD APLRKPGGYF1141AAPAQPDPDD QFIIPPELEE EVKEIMKHHQ EQQQQQQPPP PPQLQPQLPR HYQLDQLPDY1201YDTPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1205 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.