LSBio
Human SCOP / PHLPP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SCOP / PHLPP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PHLPP1 antibodies →
$153.00each
Catalog Number: LS-G79977-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PHLPP(PHLPP1) (NM_194449) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSCOP | KIAA0606 | PHLPP1 | PLEKHE1 | SCOP | PHLPP
Origin Species
Human
NCBI GenBank
AB011178
SwissProt
O60346

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
184.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSEIGCLIR FYAGKPHSTG SSERIQLSGM YNVRKGKMQL PVNRWTRRQV ILCGTCLIVS
61SVKDSLTGKM HVLPLIGGKV EEVKKHQHCL AFSSSGPQSQ TYYICFDTFT EYLRWLRQVS
121KVASQRISSV DLSCCSLEHL PANLFYSQDL THLNLKQNFL RQNPSLPAAR GLNELQRFTK
181LKSLNLSNNH LGDFPLAVCS IPTLAELNVS CNALRSVPAA VGVMHNLQTF LLDGNFLQSL
241PAELENMKQL SYLGLSFNEF TDIPEVLEKL TAVDKLCMSG NCVETLRLQA LRKMPHIKHV
301DLRLNVIRKL IADEVDFLQH VTQLDLRDNK LGDLDAMIFN NIEVLHCERN QLVTLDICGY
361FLKALYASSN ELVQLDVYPV PNYLSYMDVS RNRLENVPEW VCESRKLEVL DIGHNQICEL
421PARLFCNSSL RKLLAGHNQL ARLPERLERT SVEVLDVQHN QLLELPPNLL MKADSLRFLN
481ASANKLESLP PATLSEETNS ILQELYLTNN SLTDKCVPLL TGHPHLKILH MAYNRLQSFP
541ASKMAKLEEL EEIDLSGNKL KAIPTTIMNC RRMHTVIAHS NCIEVFPEVM QLPEIKCVDL
601SCNELSEVTL PENLPPKLQE LDLTGNPRLV LDHKTLELLN NIRCFKIDQP STGDASGAPA
661VWSHGYTEAS GVKNKLCVAA LSVNNFCDNR EALYGVFDGD RNVEVPYLLQ CTMSDILAEE
721LQKTKNEEEY MVNTFIVMQR KLGTAGQKLG GAAVLCHIKH DPVDPGGSFT LTSANVGKCQ
781TVLCRNGKPL PLSRSYIMSC EEELKRIKQH KAIITEDGKV NGVTESTRIL GYTFLHPSVV
841PRPHVQSVLL TPQDEFFILG SKGLWDSLSV EEAVEAVRNV PDALAAAKKL CTLAQSYGCH
901DSISAVVVQL SVTEDSFCCC ELSAGGAVPP PSPGIFPPSV NMVIKDRPSD GLGVPSSSSG
961MASEISSELS TSEMSSEVGS TASDEPPPGA LSENSPAYPS EQRCMLHPIC LSNSFQRQLS
1021SATFSSAFSD NGLDSDDEEP IEGVFTNGSR VEVEVDIHCS RAKEKEKQQH LLQVPAEASD
1081EGIVISANED EPGLPRKADF SAVGTIGRRR ANGSVAPQER SHNVIEVATD APLRKPGGYF
1141AAPAQPDPDD QFIIPPELEE EVKEIMKHHQ EQQQQQQPPP PPQLQPQLPR HYQLDQLPDY
1201YDTPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1205 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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