Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RTP5 / CXXC11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105165-100
Catalog Number: LS-G105165
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CXXC11(RTP5) (NM_173821), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C2orf85 | CXXC11 | CXXC finger protein 11
- Origin Species
- Human
- NCBI GenBank
- NM_173821
- SwissProt
- Q14D33
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRAGADMWA STFTLAMAER KPQDVWVLLP EHSLVPGCLD GGGVQYLLVG LSRLQCGHCP61GTWDSAHVHV LFHLWWDRAS HRGLVKMRIW GQRCRLCPAP EDCQVRPPGE QPFLSRLVLH121ILQDCYGDGP GPARHPREAY EGCCEACELG VCFLQKAPDP AWSANATKGN FPATAWGGTG181TVSRGKPLST PGDDLGKGGV VIAIPFSLVG TSNDQVPIAE GPAPPAGASL PVTGSCEALV241IGQGSIFLSG DSVAMPGGKG FPVAIGDPLF HGPGLLGSSI QTFELKGFLF KGRGSLCSPV301GVAQGWGPIS LNNGLVPVGK HTPTVFYCVG LSASGEGSLT FPSSLTSIFT NTLSEPTDGP361VATKEASITF PFIFTDVKDA VAEVAEGNGK EGGGQGLVPV GHDALPETNA GGLPSQVKGS421LALPFPADVQ GKDAFTDITE GKEKEGGLVT AGHDAPLEAN AEGPITVSEG CITIPFAVFD481VIKRKGGGHV AYGPQGNGCF SQGYYQKRQL RSRFHKARCG CRREEDERPG RACRRPHAEP541YEDFWIWVSM TVCVFWLMCM CRLNPGIYPQ QVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
572 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.