LSBio
Human RTP5 / CXXC11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human RTP5 / CXXC11 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G94920-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CXXC11(RTP5) (NM_173821) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2orf85 | CXXC11 | CXXC finger protein 11
Origin Species
Human
NCBI GenBank
NM_173821
SwissProt
Q14D33

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MDRAGADMWA STFTLAMAER KPQDVWVLLP EHSLVPGCLD GGGVQYLLVG LSRLQCGHCP
61GTWDSAHVHV LFHLWWDRAS HRGLVKMRIW GQRCRLCPAP EDCQVRPPGE QPFLSRLVLH
121ILQDCYGDGP GPARHPREAY EGCCEACELG VCFLQKAPDP AWSANATKGN FPATAWGGTG
181TVSRGKPLST PGDDLGKGGV VIAIPFSLVG TSNDQVPIAE GPAPPAGASL PVTGSCEALV
241IGQGSIFLSG DSVAMPGGKG FPVAIGDPLF HGPGLLGSSI QTFELKGFLF KGRGSLCSPV
301GVAQGWGPIS LNNGLVPVGK HTPTVFYCVG LSASGEGSLT FPSSLTSIFT NTLSEPTDGP
361VATKEASITF PFIFTDVKDA VAEVAEGNGK EGGGQGLVPV GHDALPETNA GGLPSQVKGS
421LALPFPADVQ GKDAFTDITE GKEKEGGLVT AGHDAPLEAN AEGPITVSEG CITIPFAVFD
481VIKRKGGGHV AYGPQGNGCF SQGYYQKRQL RSRFHKARCG CRREEDERPG RACRRPHAEP
541YEDFWIWVSM TVCVFWLMCM CRLNPGIYPQ QV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

572 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.