Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RTN4 / Nogo Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100957-100
Catalog Number: LS-G100957
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Nogo A(RTN4) (NM_007008), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Human NogoA | My043 protein | Neurite outgrowth inhibitor | NOGOC | NSP-CL | Nogo protein | KIAA0886 | Reticulon 5 | Reticulon-4 | Reticulon-5 | Reticulon 4 | RTN4-B1 | RTN-X | RTN4-A | RTN4-B2 | RTN4-C | Nbla00271 | Neurite growth inhibitor 220 | NI220/250 | NOGO-A | Nogo-C | ASY | Foocen | Nbla10545 | NOGO | Nogo-B | NSP | RTN4
- Origin Species
- Human
- NCBI GenBank
- NM_020532
- SwissProt
- Q9NQC3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 22.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDGQKKNWKD KVVDLLYWRD IKKTGVVFGA SLFLLLSLTV FSIVSVTAYI ALALLSVTIS61FRIYKGVIQA IQKSDEGHPF RAYLESEVAI SEELVQKYSN SALGHVNCTI KELRRLFLVD121DLVDSLKFAV LMWVFTYVGA LFNGLTLLIL ALISLFSVPV IYERHQAQID HYLGLANKNV181KDAMAKIQAK IPGLKRKAETRTRPLEQKLISEEDLAANDILDYKDDDDKV
199 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.