RTN4 / Nogo Antibody
Catalog Number: LS-C807245
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RTN4 / Nogo Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G83837-20
Catalog Number: LS-G83837
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Nogo A(RTN4) (NM_207520) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Human NogoA | My043 protein | Neurite outgrowth inhibitor | NOGOC | NSP-CL | Nogo protein | KIAA0886 | Reticulon 5 | Reticulon-4 | Reticulon-5 | Reticulon 4 | RTN4-B1 | RTN-X | RTN4-A | RTN4-B2 | RTN4-C | Nbla00271 | Neurite growth inhibitor 220 | NI220/250 | NOGO-A | Nogo-C | ASY | Foocen | Nbla10545 | NOGO | Nogo-B | NSP | RTN4
- Origin Species
- Human
- NCBI GenBank
- NM_020532
- SwissProt
- Q9NQC3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDLDQSPLV SSSDSPPRPQ PAFKYQFVRE PEDEEEEEEE EEEDEDEDLE ELEVLERKPA61AGLSAAPVPT APAAGAPLMD FGNDFVPPAP RGPLPAAPPV APERQPSWDP SPVSSTVPAP121SPLSAAAVSP SKLPEDDEPP ARPPPPPPAS VSPQAEPVWT PPAPAPAAPP STPAAPKRRG181SSGSVDETLF ALPAASEPVI RSSAVVDLLY WRDIKKTGVV FGASLFLLLS LTVFSIVSVT241AYIALALLSV TISFRIYKGV IQAIQKSDEG HPFRAYLESE VAISEELVQK YSNSALGHVN301CTIKELRRLF LVDDLVDSLK FAVLMWVFTY VGALFNGLTL LILALISLFS VPVIYERHQA361QIDHYLGLAN KNVKDAMAKI QAKIPGLKRK AETRTRPLEQKLISEEDLAANDILDYKDDDDKV
392 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
