Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RTKN / Rhotekin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102112-100
Catalog Number: LS-G102112
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RTKN (NM_033046), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Rhotekin | RTKN | RTKN1
- Origin Species
- Human
- NCBI GenBank
- AF290512
- SwissProt
- Q9BST9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQDRLHILED LNMLYIRQMA LSLEDTELQR KLDHEIRMRE GACKLLAACS QREQALEATK61SLLVCNSRIL SYMGELQRRK EAQVLGKTSR RPSDSGPPAE RSPCRGRVCI SDLRIPLMWK121DTEYFKNKGD LHRWAVFLLL QLGEHIQDTE MILVDRTLTD ISFQSNVLFA EAGPDFELRL181ELYGACVEEE GALTGGPKRL ATKLSSSLGR SSGRRVRASL DSAGGSGSSP ILLPTPVVGG241PRYHLLAHTT LTLAAVQDGF RTHDLTLASH EENPAWLPLY GSVCCRLAAQ PLCMTQPTAS301GTLRVQQAGE MQNWAQVHGV LKGTNLFCYR QPEDADTGEE PLLTIAVNKE TRVRAGELDQ361ALGRPFTLSI SNQYGDDEVT HTLQTESREA LQSWMEALWQ LFFDMSQWKQ CCDEIMKIET421PAPRKPPQAL AKQGSLYHEM AIEPLDDIAA VTDILTQREG ARLETPPPWL AMFTDQPALP481NPCSPASVAP APDWTHPLPW GRPRTFSLDA VPPDHSPRAR SVAPLPPQRS PRTRGLCSKG541QPRTWLQSPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
550 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.