RTKN / Rhotekin Antibody
Catalog Number: LS-C412146
2 sizes / from $328.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RTKN / Rhotekin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87171-20
Catalog Number: LS-G87171
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RTKN (NM_033046) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Rhotekin | RTKN | RTKN1
- Origin Species
- Human
- NCBI GenBank
- AF290512
- SwissProt
- Q9BST9
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQDRLHILED LNMLYIRQMA LSLEDTELQR KLDHEIRMRE GACKLLAACS QREQALEATK61SLLVCNSRIL SYMGELQRRK EAQVLGKTSR RPSDSGPPAE RSPCRGRVCI SDLRIPLMWK121DTEYFKNKGD LHRWAVFLLL QLGEHIQDTE MILVDRTLTD ISFQSNVLFA EAGPDFELRL181ELYGACVEEE GALTGGPKRL ATKLSSSLGR SSGRRVRASL DSAGGSGSSP ILLPTPVVGG241PRYHLLAHTT LTLAAVQDGF RTHDLTLASH EENPAWLPLY GSVCCRLAAQ PLCMTQPTAS301GTLRVQQAGE MQNWAQVHGV LKGTNLFCYR QPEDADTGEE PLLTIAVNKE TRVRAGELDQ361ALGRPFTLSI SNQYGDDEVT HTLQTESREA LQSWMEALWQ LFFDMSQWKQ CCDEIMKIET421PAPRKPPQAL AKQGSLYHEM AIEPLDDIAA VTDILTQREG ARLETPPPWL AMFTDQPALP481NPCSPASVAP APDWTHPLPW GRPRTFSLDA VPPDHSPRAR SVAPLPPQRS PRTRGLCSKG541QPRTWLQSPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
550 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
