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RSHL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RSHL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RSPH6A antibodies →
$725.00each
Catalog Number: LS-G109399-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RSPH6A (NM_030785), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Radial spokehead-like 1 | RSPH4B | RSHL1 | RSP4 | RSP6 | RSPH6A
Origin Species
Human
NCBI GenBank
NM_030785
SwissProt
Q9H0K4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDLPPYPER PAQQPPGRRT SQASQRRHSR DQAQALAADP EERQQIPPDA QRNAPGWSQR
61GSLSQQENLL MPQVFQAEEA RLGGMEYPSV NTGFPSEFQP QPYSDESRMQ VAELTTSLML
121QRLQQGQSSL FQQLDPTFQE PPVNPLGQFN LYQTDQFSEG AQHGPYIRDD PALQFLPSEL
181GFPHYSAQVP EPEPLELAVQ NAKAYLLQTS INCDLSLYEH LVNLLTKILN QRPEDPLSVL
241ESLNRTTQWE WFHPKLDTLR DDPEMQPTYK MAEKQKALFT RSGGGTEGEQ EMEEEVGETP
301VPNIMETAFY FEQAGVGLSS DESFRIFLAM KQLVEQQPIH TCRFWGKILG IKRSYLVAEV
361EFREGEEEAE EEEVEEMTEG GEVMEAHGEE EGEEDEEKAV DIVPKSVWKP PPVIPKEESR
421SGANKYLYFV CNEPGLPWTR LPHVTPAQIV NARKIKKFFT GYLDTPVVSY PPFPGNEANY
481LRAQIARISA ATQVSPLGFY QFSEEEGDEE EEGGAGRDSY EENPDFEGIP VLELVDSMAN
541WVHHTQHILP QGRCTWVNPL QKTEEEEDLG EEEEKADEGP EEVEQEVGPP LLTPLSEDAE
601IMHLAPWTTR LSCSLCPQYS VAVVRSNLWP GAYAYASGKK FENIYIGWGH KYSPESFNPA
661LPAPIQQEYP SGPEIMEMSD PTVEEEQALK AAQEQALGAT EEEEEGEEEE EGEETDD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

717 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.