Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RSHL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G91985-20
Catalog Number: LS-G91985
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RSPH6A (NM_030785) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Radial spokehead-like 1 | RSPH4B | RSHL1 | RSP4 | RSP6 | RSPH6A
- Origin Species
- Human
- NCBI GenBank
- NM_030785
- SwissProt
- Q9H0K4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDLPPYPER PAQQPPGRRT SQASQRRHSR DQAQALAADP EERQQIPPDA QRNAPGWSQR61GSLSQQENLL MPQVFQAEEA RLGGMEYPSV NTGFPSEFQP QPYSDESRMQ VAELTTSLML121QRLQQGQSSL FQQLDPTFQE PPVNPLGQFN LYQTDQFSEG AQHGPYIRDD PALQFLPSEL181GFPHYSAQVP EPEPLELAVQ NAKAYLLQTS INCDLSLYEH LVNLLTKILN QRPEDPLSVL241ESLNRTTQWE WFHPKLDTLR DDPEMQPTYK MAEKQKALFT RSGGGTEGEQ EMEEEVGETP301VPNIMETAFY FEQAGVGLSS DESFRIFLAM KQLVEQQPIH TCRFWGKILG IKRSYLVAEV361EFREGEEEAE EEEVEEMTEG GEVMEAHGEE EGEEDEEKAV DIVPKSVWKP PPVIPKEESR421SGANKYLYFV CNEPGLPWTR LPHVTPAQIV NARKIKKFFT GYLDTPVVSY PPFPGNEANY481LRAQIARISA ATQVSPLGFY QFSEEEGDEE EEGGAGRDSY EENPDFEGIP VLELVDSMAN541WVHHTQHILP QGRCTWVNPL QKTEEEEDLG EEEEKADEGP EEVEQEVGPP LLTPLSEDAE601IMHLAPWTTR LSCSLCPQYS VAVVRSNLWP GAYAYASGKK FENIYIGWGH KYSPESFNPA661LPAPIQQEYP SGPEIMEMSD PTVEEEQALK AAQEQALGAT EEEEEGEEEE EGEETDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
717 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
