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RRP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RRP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RRP12 antibodies →
$725.00each
Catalog Number: LS-G126532-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RRP12 (NM_001145114), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0690 | RRP12 | RRP12-like protein
Origin Species
Human
NCBI GenBank
NM_015179
SwissProt
Q5JTH9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRSGKLPSG VSAKLKRWKK GHSSDSNPAI CRHRQAARSR FFSRPSGRSD LTVDAVKLHN
61ELQSGSLRLG KSEAPETPME EEAELVLTEK SSGTFLSGLS DCTNVTFSKV QRFWESNSAA
121HKEICAVLAA VTEVIRSQGG KETETEYFAA LVLSCLATLL RKQDLEAWGY PVTLQVYHGL
181LSFTVHPKPK IRKAAQHGVC SVLKGSEFMF EKAPAHHPAA ISTAKFCIQE IEKSGGSKEA
241TTTLHMLTLL KDLLPCFPEG LVKSCSETLL RVMTLSHVLV TACAMQAFHS LFHARPGLST
301LSAELNAQII TALYDYVPSE NDLQPLLAWL KVMEKAHINL VRLQWDLGLG HLPRFFGTAV
361TCLLSPHSQV LTAATQSLKE ILKECVAPHM ADIGSVTSSA SGPAQSVAKM FRAVEEGLTY
421KFHAAWSSVL QLLCVFFEAC GRQAHPVMRK CLQSLCDLRL SPHFPHTAAL DQAVGAAVTS
481MGPEVVLQAV PLEIDGSEET LDFPRSWLLP VIRDHVQETR LGFFTTYFLP LANTLKSKAM
541DLAQAGSTVE SKIYDTLQWQ MWTLLPGFCT RPTDVAISFK GLARTLGMAI SERPDLRVTV
601CQALRTLITK GCQAEADRAE VSRFAKNFLP ILFNLYGQPV AAGDTPAPRR AVLETIRTYL
661TITDTQLVNS LLEKASEKVL DPASSDFTRL SVLDLVVALA PCADEAAISK LYSTIRPYLE
721SKAHGVQKKA YRVLEEVCAS PQGPGALFVQ SHLEDLKKTL LDSLRSTSSP AKRPRLKCLL
781HIVRKLSAEH KEFITALIPE VILCTKEVSV GARKNAFALL VEMGHAFLRF GSNQEEALQC
841YLVLIYPGLV GAVTMVSCSI LALTHLLFEF KGLMGTSTVE QLLENVCLLL ASRTRDVVKS
901ALGFIKVAVT VMDVAHLAKH VQLVMEAIGK LSDDMRRHFR MKLRNLFTKF IRKFGFELVK
961RLLPEEYHRV LVNIRKAEAR AKRHRALSQA AVEEEEEEEE EEEPAQGKGD SIEEILADSE
1021DEEDNEEEER SRGKEQRKLA RQRSRAWLKE GGGDEPLNFL DPKVAQRVLA TQPGPGRGRK
1081KDHGFKVSAD GRLIIREEAD GNKMEEEEGA KGEDEEMADP MEDVIIRNKK HQKLKHQKEA
1141EEEELEIPPQ YQAGGSGIHR PVAKKAMPGA EYKAKKAKGD VKKKGRPDPY AYIPLNRSKL
1201NRRKKMKLQG QFKGLVKAAR RGSQVGHKNR RKDRRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1236 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.