LSBio
RRP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RRP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RRP12 antibodies →
$504.00each
Catalog Number: LS-G113402-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RRP12 (NM_015179), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0690 | RRP12 | RRP12-like protein
Origin Species
Human
NCBI GenBank
NM_015179
SwissProt
Q5JTH9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
143.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRSGKLPSG VSAKLKRWKK GHSSDSNPAI CRHRQAARSR FFSRPSGRSD LTVDAVKLHN
61ELQSGSLRLG KSEAPETPME EEAELVLTEK SSGTFLSGLS DCTNVTFSKV QRFWESNSAA
121HKEICAVLAA VTEVIRSQGG KETETEYFAA LMTTMEAVES PESLAAVAYL LNLVLKRVPS
181PVLIKKFSDT SKAFMDIMSA QASSGSTSVL RWVLSCLATL LRKQDLEAWG YPVTLQVYHG
241LLSFTVHPKP KIRKAAQHGV CSVLKGSEFM FEKAPAHHPA AISTAKFCIQ EIEKSGGSKE
301ATTTLHMLTL LKDLLPCFPE GLVKSCSETL LRVMTLSHVL VTACAMQAFH SLFHARPGLS
361TLSAELNAQI ITALYDYVPS ENDLQPLLAW LKVMEKAHIN LVRLQWDLGL GHLPRFFGTA
421VTCLLSPHSQ VLTAATQSLK EILKECVAPH MADIGSVTSS ASGPAQSVAK MFRAVEEGLT
481YKFHAAWSSV LQLLCVFFEA CGRQAHPVMR KCLQSLCDLR LSPHFPHTAA LDQAVGAAVT
541SMGPEVVLQA VPLEIDGSEE TLDFPRSWLL PVIRDHVQET RLGFFTTYFL PLANTLKSKA
601MDLAQAGSTV ESKIYDTLQW QMWTLLPGFC TRPTDVAISF KGLARTLGMA ISERPDLRVT
661VCQALRTLIT KGCQAEADRA EVSRFAKNFL PILFNLYGQP VAAGDTPAPR RAVLETIRTY
721LTITDTQLVN SLLEKASEKV LDPASSDFTR LSVLDLVVAL APCADEAAIS KLYSTIRPYL
781ESKAHGVQKK AYRVLEEVCA SPQGPGALFV QSHLEDLKKT LLDSLRSTSS PAKRPRLKCL
841LHIVRKLSAE HKEFITALIP EVILCTKEVS VGARKNAFAL LVEMGHAFLR FGSNQEEALQ
901CYLVLIYPGL VGAVTMVSCS ILALTHLLFE FKGLMGTSTV EQLLENVCLL LASRTRDVVK
961SALGFIKVAV TVMDVAHLAK HVQLVMEAIG KLSDDMRRHF RMKLRNLFTK FIRKFGFELV
1021KRLLPEEYHR VLVNIRKAEA RAKRHRALSQ AAVEEEEEEE EEEEPAQGKG DSIEEILADS
1081EDEEDNEEEE RSRGKEQRKL ARQRSRAWLK EGGGDEPLNF LDPKVAQRVL ATQPGPGRGR
1141KKDHSFKVSA DGRLIIREEA DGNKMEEEEG AKGEDEEMAD PMEDVIIRNK KHQKLKHQKE
1201AEEEELEIPP QYQAGGSGIH RPVAKKAMPG AEYKAKKAKG DVKKKGRPDP YAYIPLNRSK
1261LNRRKKMKLQ GQFKGLVKAA RRGSQVGHKN RRKDRRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1297 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.